The trehalose-6-phosphate synthase TPS5 negatively regulates ABA signaling in Arabidopsis thaliana
The trehalose-6-phosphate synthase TPS5 negatively regulates ABA signaling in Arabidopsis thaliana
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海藻糖-6-磷酸合酶 TPS5 负向调节拟南芥中的 ABA 信号传导
DOI：
10.1007/s00299-019-02408-y
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发表时间：
2019-04
影响因子：
6.2
通讯作者：
Chen Liangbi
中科院分区：
文献类型：
--
作者：
Tian Lianfu;Xie Zijing;Lu Changqing;Hao Xiaohua;Wu Sha;Huang Yuan;Li Dongping;Chen Liangbi
在拟南芥种子萌发和气孔关闭过程中，TPS5 通过介导 ROS 水平和 NR 活性来负向调节 ABA 信号传导。海藻糖代谢对于植物生长发育以及非生物胁迫反应很重要。在拟南芥中鉴定出11个TPS基因，分为I类（TPS1-TPS4）和II类（TPS5-TPS11）。尽管 I 类已被证明具有 TPS 活性，但 II 类的大多数成员的功能仍然是个谜。在这里，我们表征了拟南芥中海藻糖-6-磷酸合酶 TPS5 在 ABA 信号传导中的生物学功能。 TPS5 表达由 ABA 和非生物胁迫诱导，并且在 ABA 处理后表皮和保卫细胞中的表达显着增加。功能缺失分析表明，tps5 突变体（tps5-1 和 tps5-cas9）在种子萌发和 ABA 介导的气孔关闭过程中对 ABA 更敏感。此外，tps5-1和tps5-cas9突变体中H2O2水平增加，这与负责H2O2产生的关键基因RbohD和RbohF表达的变化一致。此外，TPS5 敲除降低了海藻糖和其他可溶性碳水化合物的含量以及硝酸还原酶 (NR) 活性。在体外，海藻糖和其他可溶性碳水化合物促进 NR 活性，但该活性被三羧酸循环抑制剂碘乙酸阻断。因此，本研究确定 TPS5 作为 ABA 信号传导的负调节因子，并参与改变海藻糖含量和 NR 活性。
The TPS5 negatively regulates ABA signaling by mediating ROS level and NR activity during seed germination and stomatal closure in Arabidopsis thaliana. Trehalose metabolism is important in plant growth and development and in abiotic stress response. Eleven TPS genes were identified in Arabidopsis, divided into Class I (TPS1-TPS4) and Class II (TPS5-TPS11). Although Class I has been shown to have TPS activity, the function of most members of Class II remains enigmatic. Here, we characterized the biological function of the trehalose-6-phosphate synthase TPS5 in ABA signaling in Arabidopsis. TPS5 expression was induced by ABA and abiotic stress, and expression in epidermal and guard cells was dramatically increased after ABA treatment. Loss-of-function analysis revealed that tps5 mutants (tps5-1 and tps5-cas9) are more sensitive to ABA during seed germination and ABA-mediated stomatal closure. Furthermore, the H2O2level increased in the tps5-1 and tps5-cas9 mutants, which was consistent with the changes in the expression of RbohD and RbohF, key genes responsible for H2O2production. Further, TPS5 knockout reduced the amounts of trehalose and other soluble carbohydrates as well as nitrate reductase (NR) activity. In vitro, trehalose and other soluble carbohydrates promoted NR activity, which was blocked by the tricarboxylic acid cycle inhibitor iodoacetic acid. Thus, this study identified that TPS5 functions as a negative regulator of ABA signaling and is involved in altering the trehalose content and NR activity.
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影响因子：
7.4
作者：
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通讯作者：
Emmanuelle Pigaglio;N. Durand;Christian Meyer
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
5.6
作者：
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通讯作者：
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影响因子：
11.6
作者：
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通讯作者：
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