RPS9M, a Mitochondrial Ribosomal Protein, Is Essential for Central Cell Maturation and Endosperm Development in Arabidopsis.
RPS9M, a Mitochondrial Ribosomal Protein, Is Essential for Central Cell Maturation and Endosperm Development in Arabidopsis.
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RPS9M 是一种线粒体核糖体蛋白，对于拟南芥中央细胞成熟和胚乳发育至关重要
DOI：
10.3389/fpls.2017.02171
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发表时间：
2017
影响因子：
5.6
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Lu C;Yu F;Tian L;Huang X;Tan H;Xie Z;Hao X;Li D;Luan S;Chen L
在被子植物的双受精过程中，雌配子体的中央细胞与精细胞融合产生胚乳，胚乳是一种将发育中的胚包裹在种子内的储存组织。虽然许多遗传突变体缺陷的女性配子体功能的特点，控制中央细胞的规格和分化的分子机制仍然没有完全理解。在这里，我们报告的线粒体核糖体蛋白，RPS9M，是中央细胞成熟所必需的。RPS9M在双受精前后的雄配子体和雌配子体中都有高表达。雌性配子体在rps9m突变体中有缺陷，特别是关于中央细胞的成熟。形态学缺陷包括极核不融合和中央细胞内中央空泡变小。此外，rps9m雌配子体的胚起始和早期胚乳发育也受到严重影响，即使在与野生型花粉受精后。RPS9M可以与ANK6相互作用，ANK6是线粒体中的一种锚定重复蛋白，此前报道是受精所必需的。RPS9M的表达模式和突变体表型与ANK6相似，提示RPS9M可能与ANK6共同调控雌配子体发育，可能是通过调节线粒体蛋白的表达。
During double fertilization of angiosperms, the central cell of the female gametophyte fuses with a sperm cell to produce the endosperm, a storage tissue that nourishes the developing embryo within the seed. Although many genetic mutants defective in female gametophytic functions have been characterized, the molecular mechanisms controlling the specification and differentiation of the central cell are still not fully understood. Here, we report a mitochondrial ribosomal protein, RPS9M, is required for central cell maturation. RPS9M was highly expressed in the male and female gametophytes before and after double fertilization. The female gametophytes were defective in the rps9m mutant specifically concerning maturation of central cells. The morphological defects include unfused polar nuclei and smaller central vacuole in central cells. In addition, embryo initiation and early endosperm development were also severely affected in rps9m female gametophytes even after fertilized with wild type pollens. The RPS9M can interact with ANK6, an ankyrin-repeat protein in mitochondria previously reported to be required for fertilization. The expression pattern and mutant phenotype of RPS9M are similar to those of ANK6 as well, suggesting that RPS9M may work together with ANK6 in controlling female gametophyte development, possibly by regulating the expression of some mitochondrial proteins.
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影响因子：
13.8
作者：
Gilbert, Wendy V.
通讯作者：
Gilbert, Wendy V.
影响因子：
7.4
作者：
Falcone Ferreyra, Maria Lorena;Pezza, Alejandro;Casati, Paula
通讯作者：
Casati, Paula
影响因子：
10.7
作者：
Bonen, L;Calixte, S
通讯作者：
Calixte, S
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
--
作者：
Christensen, CA;King, EJ;Drews, GN
通讯作者：
Drews, GN