Nod1, but not the ASC inflammasome, contributes to induction of IL-1β secretion in human trophoblasts after sensing of Chlamydia trachomatis.
Nod1, but not the ASC inflammasome, contributes to induction of IL-1β secretion in human trophoblasts after sensing of Chlamydia trachomatis.
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作者：
沙眼衣原体（Chlamydia trachomatis，Ct）是一种专性胞内致病菌。以前，我们发现Ct感染人滋养层细胞会触发促炎细胞因子IL-1β的分泌。本研究旨在了解Ct感染后滋养细胞产生IL-1β的天然免疫途径。我们采取的方法是抑制与衣原体感染相关的关键Toll样受体（TLR）、Nod样受体（NLR）和炎性小体组分的表达或功能。在本研究中，我们报告了Ct诱导的滋养细胞IL-1β分泌与IL-1β mRNA的转录、IL-1β前体的翻译和加工以及caspase-1的激活有关。此外，我们证明了Ct诱导的滋养层IL-1β的产生和分泌不依赖于TLR 2、TLR 4、MyD 88和Nalp 3/ASC炎性体。相反，我们报告，第一次，Nod 1介导滋养层IL-1β分泌的重要性，响应Ct感染。
Chlamydia trachomatis (Ct) is an obligate intracellular bacterial pathogen. Previously, we showed that infection of human trophoblast cells by Ct triggers the secretion of the pro-inflammatory cytokine, IL-1β. The aim of this study was to understand the innate immune pathways involved in trophoblast production of IL-1β after Ct infection. The approach we took was to inhibit the expression or function of the key Toll-like receptors (TLRs), Nod-like receptors (NLRs) and inflammasome components that have been associated with chlamydia infection. In this study we report that Ct-induced trophoblast IL-1β secretion is associated with the transcription of IL-1β mRNA, the translation and processing of pro-IL-1β, and the activation of caspase-1. In addition, we demonstrate that Ct-induced IL-1β production and secretion by the trophoblast is independent of TLR2, TLR4, MyD88, and the Nalp3/ASC inflammasome. Instead we report, for the first time, the importance of Nod1 for mediating trophoblast IL-1β secretion in response to a Ct infection.
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DOI：
10.4049/jimmunol.0903937
发表时间：
2010-05-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
He X;Mekasha S;Mavrogiorgos N;Fitzgerald KA;Lien E;Ingalls RR
通讯作者：
Ingalls RR
影响因子：
4.4
作者：
Cardenas, Ingrid;Mulla, Melissa J.;Abrahams, Vikki M.
通讯作者：
Abrahams, Vikki M.
影响因子：
3.1
作者：
Buchholz, Kerry R.;Stephens, Richard S.
通讯作者：
Stephens, Richard S.
影响因子：
3.4
作者：
Abrahams, Vikki M.
通讯作者：
Abrahams, Vikki M.
影响因子：
32.4
作者：
Allen, Irving C.;Scull, Margaret A.;Moore, Chris B.;Holl, Eda K.;McElvania-TeKippe, Erin;Taxman, Debra J.;Guthrie, Elizabeth H.;Pickles, Raymond J.;Ting, Jenny P. -Y.
通讯作者：
Ting, Jenny P. -Y.