OsLMP1, Encoding a Deubiquitinase, Regulates the Immune Response in Rice.
OsLMP1, Encoding a Deubiquitinase, Regulates the Immune Response in Rice.
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OsLMP1，编码去泛素酶，调节水稻的免疫反应
DOI：
10.3389/fpls.2021.814465
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发表时间：
2021
影响因子：
5.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Sun J;Song W;Chang Y;Wang Y;Lu T;Zhang Z
病变模拟突变体已成为了解植物与微生物相互作用和植物免疫机制的有效材料。虽然在植物中已经阐明了许多引起病变模仿表型的机制，但病变模仿由翻译后修饰调节的机制仍然很大程度上是难以理解的，特别是在水稻中。在这项研究中，获得了一个具有病变模拟表型的突变体，并命名为lmp1-1。生理测量和实时荧光定量PCR分析表明，突变体的防御反应被激活。转录组分析表明，突变体中苯丙氨酸解氨酶（PAL）通路被激活，导致水杨酸（SA）积累。基于定位克隆的结果显示，OsLMP1编码一个去泛素酶。OsLMP1可以切割泛素化前体。此外，OsLMP1通过去泛素化H2B修饰SA合成途径基因，调控水稻的免疫应答。综上所述，本研究加深了我们对OsLMP1在植物免疫应答中的功能的认识，并为植物与病原微生物之间的关系提供了进一步的认识。
Lesion mimic mutants have become an effective material for understanding plant-microbe interactions and the immune mechanism in plants. Although many mechanisms responsible for the lesion mimic phenotype have been clarified in plants, the mechanism by which lesion mimic is regulated by posttranslational modification remained largely elusive, especially in rice. In this study, a mutant with the lesion mimic phenotype was obtained and named lmp1-1. Physiological measurements and quantitative real-time PCR analysis showed that the defense response was activated in the mutants. Transcriptome analysis showed that the phenylalanine ammonia lyase (PAL) pathway was activated in the mutant, causing the accumulation of salicylic acid (SA). The results of mapping based cloning showed that OsLMP1 encodes a deubiquitinase. OsLMP1 can cleave ubiquitination precursors. Furthermore, OsLMP1 epigenetically modifies SA synthetic pathway genes by deubiquitinating H2B and regulates the immune response in rice. In summary, this study deepens our understanding of the function of OsLMP1 in the plant immune response and provides further insight into the relationship between plants and pathogenic microorganisms.
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影响因子：
4.5
作者：
Gao H;Zheng XM;Fei G;Chen J;Jin M;Ren Y;Wu W;Zhou K;Sheng P;Zhou F;Jiang L;Wang J;Zhang X;Guo X;Wang JL;Cheng Z;Wu C;Wang H;Wan JM
通讯作者：
Wan JM
影响因子：
6.9
作者：
Jubany-Marí T;Munné-Bosch S;López-Carbonell M;Alegre L
通讯作者：
Alegre L
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影响因子：
56.9
作者：
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通讯作者：
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影响因子：
7.3
作者：
Keren, Ido;Citovsky, Vitaly
通讯作者：
Citovsky, Vitaly
影响因子：
7.4
作者：
Bouchez, Olivier;Huard, Carine;Balague, Claudine
通讯作者：
Balague, Claudine