Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi.
Investigation of the Sandfly Fauna of Central Arid Areas and Northern Humid Regions of Tunisia, with Morphological and Molecular Identification of the Recently Established Population of Phlebotomus (Larroussius) perfiliewi.
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Perfiliewi白蛉是包括突尼斯在内的西地中海盆地婴儿利什曼原虫的主要媒介之一，婴儿利什曼原虫是人畜共患内脏利什曼病（ZVL）的病原体。白蛉perfiliewi，原来只在北方潮湿的生物气候带，成为建立在中央干旱生物气候带的高度灌溉区。因此，白蛉病媒的监测是制定控制战略的基石。昆虫学调查进行了使用光陷阱在潮湿和高度灌溉的干旱生物气候带位于突尼斯北方和中部，分别。根据形态学标准对采集的白蛉进行鉴定，并通过分子工具确认了对白蛉的鉴定。结果表明，在这两个地点的白蛉物种最丰富的是Ph.perfiliewi，它是遗传变异。因此，迫切需要监测和控制白蛉，以限制白蛉传播疾病的发生。基于核苷酸序列，我们重新确定了代表性样品的白蛉perfiliewi来自两个不同的地理区域的突尼斯，其人口以前已确定的基础上形态标准。靶向线粒体DNA细胞色素B基因的部分区域，并通过BLAST分析，考虑查询覆盖率和同一性百分比，通过与GenBank数据库中可用的DNA序列类比来确定白蛉物种。所识别的物种与所分析的序列具有最大的同源性。分子鉴定结果与形态学鉴定结果完全一致，全白白蛉具有遗传变异性。
Phlebotomus perfiliewi is one of the main vectors of Leishmania infantum, etiologic agent of zoonotic visceral leishmaniasis (ZVL), in the Western Mediterranean basin including Tunisia. Phlebotomus perfiliewi, originally endemic only in Northern humid bio-climatic zones, became established in a highly irrigated area of the central arid bioclimatic zone. Therefore, surveillance of sandfly vectors is a cornerstone for the establishment of control strategies. An entomological survey was carried out using light traps in a humid and in a highly irrigated arid bio-climatic zone located in northern and central Tunisia, respectively. Collected sandflies were identified based on morphological criteria, and identification of Ph. perfiliewi was confirmed by molecular tools. Results indicated that Ph. perfiliewi is the most abundant sandfly species in both sites and it is genetically variable. Thus, surveillance and control of sandflies is highly needed to limit the incidence of sandfly-borne diseases. Based on nucleotide sequences, we re-identified representative samples of Phlebotomus perfiliewi originating from two different biogeographical areas of Tunisia, whose populations had previously been identified based on morphological criteria. A partial region of the mitochondrial DNA cytochrome b gene was targeted, and sandfly species was determined by analogy with DNA sequences available in the GenBank database via a BLAST analysis, taking into account the query coverage and percentage identity. The recognized species presents the most substantial homology with the analyzed sequence. The results of the molecular identification showed complete agreement with the morphological identifications, and Phlebotomus perfiliewi is genetically variable.
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影响因子：
4
作者：
Depaquit, J;Ferté, H;Volf, P
通讯作者：
Volf, P
影响因子：
--
作者：
Barhoumi, W.;Chelbi, I.;Zhioua, E.
通讯作者：
Zhioua, E.
影响因子：
3.8
作者：
Bennai, Kahina;Tahir, Djamel;Parola, Philippe
通讯作者：
Parola, Philippe
影响因子：
2.9
作者：
Ghrab, J;Rhim, A;Bouratbine, A
通讯作者：
Bouratbine, A
DOI：
10.3390/pathogens11080855
发表时间：
2022-07-29
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
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