β3 integrin immunohistochemistry as a method to predict sentinel lymph node status in patients with primary cutaneous melanoma.
β3 integrin immunohistochemistry as a method to predict sentinel lymph node status in patients with primary cutaneous melanoma.
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DOI：
10.1111/ijd.15125
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发表时间：
2020-10
影响因子：
3.6
通讯作者：
Meves A
中科院分区：
文献类型：
--
作者：
Quattrocchi E;Sominidi-Damodaran S;Murphree DH;Meves A
整合素是由非共价连接的 α 和 β 亚基组成的异二聚体蛋白质，对于广泛的正常生理学至关重要，并且在癌症中也发挥着重要作用。在这里，我们测试了诊断性皮肤活检中整合素的表达是否与前哨淋巴结（SLN）转移相关。我们利用 854 名连续原发性皮肤黑色素瘤患者的队列，通过逆转录酶定量 PCR (RT-qPCR) 来量化 β 整合素亚基的表达。此外，我们通过免疫组织化学 (IHC) 通过 H 评分对 271 名患者的亚组中 β3 整合素的表达进行了定量。感兴趣的结果是黑色素瘤诊断后 90 天内 SLN 活检转移。使用逻辑回归分析根据分子、临床和组织学变量开发 SLN 转移可能性的模型。通过 IHC 或 RT-qPCR 定量的 β3 整合素表达与 SLN 转移相关。 β1、β5、β6 和 β8 整合素表达与 SLN 转移无关。预测模型的性能增量有限，该模型包括通过 IHC 量化的 β3 整合素表达以及已建立的临床病理变量（Breslow 深度和患者年龄）。 β3 整合素是与原发性皮肤黑色素瘤前哨淋巴结活检 (SLNb) 转移相关的主要整合素亚基。然而，相对于 Breslow 深度和患者年龄，β3 整合素 H 评分并不能显着改善 SLN 转移可能性的模型。
Integrins are heterodimeric proteins composed of noncovalently linked α and β subunits which are essential for a wide range of normal physiology and also play prominent roles in cancer. Here we tested whether integrin expression in diagnostic skin biopsies is associated with sentinel lymph node (SLN) metastasis. We utilized a cohort of 854 consecutive patients with primary cutaneous melanoma to quantify the expression of β integrin subunits by reverse transcriptase quantitative PCR (RT-qPCR). In addition, we quantified the expression of β3 integrin by immunohistochemistry (IHC) in a subset of 271 patients by H score. Outcome of interest was SLN biopsy metastasis within 90 days of melanoma diagnosis. Logistic regression analyses were used to develop models for the likelihood of SLN metastasis from molecular, clinical, and histologic variables. β3 integrin expression quantified by IHC or RT-qPCR was associated with SLN metastasis. β1, β5, β6, and β8 integrin expression was not associated with SLN metastasis. The incremental gain in performance of a predictive model which included β3 integrin expression as quantified by IHC in combination with established clinicopathologic variables (Breslow depth and patient age) was limited. β3 integrin is the principal integrin subunit associated with sentinel lymph node biopsy (SLNb) metastasis in primary cutaneous melanoma. However, β3 integrin H score does not significantly improve models for the likelihood of SLN metastasis over Breslow depth and patient age.
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DOI：
10.1148/rg.2017160130
发表时间：
2017-03
期刊：
Radiographics : a review publication of the Radiological Society of North America, Inc
影响因子：
--
作者：
Erickson BJ;Korfiatis P;Akkus Z;Kline TL
通讯作者：
Kline TL
影响因子：
3.6
作者：
Grossman, Douglas;Kim, Caroline C.;Swetter, Susan M.
通讯作者：
Swetter, Susan M.
影响因子：
6.4
作者：
DANEN, EHJ;TENBERGE, PJM;RUITER, DJ
通讯作者：
RUITER, DJ
影响因子：
45.3
作者：
Meves, Alexander;Nikolova, Ekaterina;Suman, Vera J.
通讯作者：
Suman, Vera J.
影响因子：
11.5
作者：
Gerami, Pedram;Cook, Robert W.;Stone, John F.
通讯作者：
Stone, John F.