The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes.
The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes.
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DOI：
10.3390/bioengineering10091000
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发表时间：
2023-08-24
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影响因子：
--
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尽管干燥技术是冷冻保存的令人兴奋的替代方案，但在干燥产品的储存期间保持严格控制的温度和湿度水平仍然具有挑战性。本研究的目的是确定向海藻糖溶液中添加乙酸胆碱是否可以通过允许可能导致海藻糖结晶和DNA完整性丧失的暂时湿度偏移（>44%相对湿度），实现更广泛的储存条件，以保存完全生长的卵母细胞的核。以家猫生殖囊泡卵母细胞为模型，研究了微波辅助脱水后卵母细胞的复苏率和完整性。单独暴露于醋酸胆碱不会损害germinal囊泡的DNA完整性，只会对染色质构型产生负面影响。添加乙酸胆碱使我们能够在微波辅助干燥25分钟后达到较低的水分含量。再水化后的样品回收率在乙酸胆碱的存在下也更好。的完整性的胚芽囊泡的DNA没有受到影响，而染色质的配置受损的存在下，在脱水过程中的乙酸胆碱。重要的是，乙酸胆碱的加入有助于在偏离理想湿度条件的过程中保持无定形状态（不存在有害的结晶）。
Although drying techniques are exciting alternatives to cryopreservation, it remains challenging to maintain tightly controlled temperatures and humidity levels during storage of dried products. The objective of this study was to determine if the addition of choline acetate to trehalose solution could enable a wider range of storage conditions for preservation of nuclei from fully grown oocytes, by allowing temporary humidity excursions (>44% relative humidity) that may lead to crystallization of trehalose and loss of DNA integrity. Using domestic cat germinal vesicle oocytes as a model, we characterized the recovery as well as the integrity of samples after microwave-assisted dehydration. Exposure to choline acetate alone did not impair the germinal vesicle’s DNA integrity and only had a negative impact on the chromatin configuration. Choline acetate addition enabled us to reach lower moisture contents after 25 min of microwave-assisted drying. Sample recovery after rehydration was also better in the presence of choline acetate. The integrity of the germinal vesicle’s DNA was not affected, while the chromatin configuration was impaired by the presence of choline acetate during dehydration. Importantly, choline acetate addition helped to maintain an amorphous state (absence of detrimental crystallization) during excursion from ideal humidity conditions.
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影响因子：
1.6
作者：
Elliott, Gloria D.;Lee, Pei-Chih;Comizzoli, Pierre
通讯作者：
Comizzoli, Pierre
影响因子：
2.7
作者：
Mazzobre, MF;Longinotti, MP;Buera, MP
通讯作者：
Buera, MP
影响因子：
2.8
作者：
Patrick, Jennifer L.;Elliott, Gloria D.;Comizzoli, Pierre
通讯作者：
Comizzoli, Pierre
影响因子：
2.8
作者：
Tharasanit, Theerawat;Manee-In, Sukanya;Techakumphu, Mongkol
通讯作者：
Techakumphu, Mongkol
影响因子：
3.2
作者：
Colombo M;Zahmel J;Jänsch S;Jewgenow K;Luvoni GC
通讯作者：
Luvoni GC