Use of bioluminescent bacterial biosensors to investigate the role of free-living helminths as reservoirs and vectors of Salmonella.

Use of bioluminescent bacterial biosensors to investigate the role of free-living helminths as reservoirs and vectors of Salmonella.
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使用生物发光细菌生物传感器研究自由生活的蠕虫作为沙门氏菌储存库和载体的作用。

DOI:
10.1111/j.1758-2229.2009.00031.x
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发表时间:
2009
影响因子:
3.3
通讯作者:
Lacharme-Lora L
Lacharme-Lora L
中科院分区:
生物学3区
文献类型:
--
作者:
Lacharme-Lora L

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自由生活的食微生物蠕虫消耗病原菌,可以为这些病原体提供环境避难所,并且在意外摄入的情况下,可以将食源性病原体传播给人类和牲畜。我们在一系列模拟恶劣环境条件的实验中,通过比较被秀丽隐杆线虫摄入的沙门氏菌与单独细菌的存活率来验证这一假设。利用luxgene技术记录沙门氏菌在体内的生长情况,发现在C.在pH2和pH3条件下,在氯的存在下,当暴露于紫外线照射时,沙门氏菌表现出增强的存活率,从而为细菌提供了环境避难所或储存库。在感染C.在用生物发光沙门氏菌喂养的线虫中,真实的实时成像显示动物发生了全身性细菌感染,这表明自由生活的蠕虫可以作为病原体的载体发挥作用。
Free‐living microbivorous helminths that consume pathogenic bacteria could offer an environmental refuge for those pathogens and also, in the case of accidental ingestion, could transmit food‐borne pathogens to humans and livestock. We tested this hypothesis by comparing the survival ofSalmonellabacteria that had been ingested by the helminthCaenorhabditis eleganswith that of the bacteria alone, in a series of experiments to mimic harsh environmental conditions. Usingluxgene technology to record thein vivogrowth ofSalmonellawe found that when insideC. elegans, theSalmonellaexhibited enhanced survival at pH 2 and 3, in the presence of chlorine and when exposed to UV irradiation, thereby providing an environmental refuge or reservoir for the bacteria. On inoculating laboratory mice withC. elegansthat had been fed on bioluminescentSalmonella, real‐time imaging showed that animals developed a systemic bacterial infection, indicating that free‐living helminths could play a role as a vector of pathogens.
新鲜蔬菜线虫、变形虫和沙门氏菌调查。
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