Use of bioluminescent bacterial biosensors to investigate the role of free-living helminths as reservoirs and vectors of Salmonella.
Use of bioluminescent bacterial biosensors to investigate the role of free-living helminths as reservoirs and vectors of Salmonella.
复制标题
使用生物发光细菌生物传感器研究自由生活的蠕虫作为沙门氏菌储存库和载体的作用。
DOI:
10.1111/j.1758-2229.2009.00031.x
复制
发表时间:
2009
影响因子:
3.3
通讯作者:
Lacharme-Lora L
中科院分区:
文献类型:
--
作者:
Lacharme-Lora L
Free‐living microbivorous helminths that consume pathogenic bacteria could offer an environmental refuge for those pathogens and also, in the case of accidental ingestion, could transmit food‐borne pathogens to humans and livestock. We tested this hypothesis by comparing the survival ofSalmonellabacteria that had been ingested by the helminthCaenorhabditis eleganswith that of the bacteria alone, in a series of experiments to mimic harsh environmental conditions. Usingluxgene technology to record thein vivogrowth ofSalmonellawe found that when insideC. elegans, theSalmonellaexhibited enhanced survival at pH 2 and 3, in the presence of chlorine and when exposed to UV irradiation, thereby providing an environmental refuge or reservoir for the bacteria. On inoculating laboratory mice withC. elegansthat had been fed on bioluminescentSalmonella, real‐time imaging showed that animals developed a systemic bacterial infection, indicating that free‐living helminths could play a role as a vector of pathogens.
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DOI:
--
发表时间:
1984
期刊:
Journal - Association of Official Analytical Chemists
影响因子:
--
作者:
R. A. Rude;G. J. Jackson;J. W. Bier;T. Sawyer;N. G. Risty
通讯作者:
N. G. Risty
影响因子:
2.6
作者:
J. Cabaret;N. Mangeon;L. Gruner
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作者:
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Berk, Sharon G.
影响因子:
2.1
作者:
S. Nelson;A. Cooper;E. L. Taylor;V. Salisbury
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V. Salisbury
DOI:
--
发表时间:
2006
期刊:
International Journal of Parasitology
影响因子:
--
作者:
S. Perkins;A. Fenton
通讯作者:
A. Fenton