High-density microfibers as a potential optical interface to reach deep brain regions.
High-density microfibers as a potential optical interface to reach deep brain regions.
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DOI：
10.1088/1741-2552/aadbb2
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发表时间：
2018-12
影响因子：
4
通讯作者：
Gardner TJ
中科院分区：
文献类型：
--
作者：
Perkins LN;Semu D;Shen J;Boas DA;Gardner TJ
由于光散射的原因，用于记录和操纵神经活动的光学技术传统上被限制在大脑表面区域。需要新的技术来将光学访问扩展到脑深部区域的大型3D体积，同时保持局部连接。我们已经开发出一种方法来植入数百或数千束光学微纤维，每束直径为8 μm。在插入过程中，每根纤维独立移动，遵循阻力最小的路径。光纤实现近全内反射，使得能够与每个光纤孔附近的组织光学地对接。在3mm的深度处，组织学显示纤维在整个目标区域中直径一致地张开超过1mm。慢性植入后的免疫组织化学染色显示神经元靠近纤维尖端。光子注量模型表明，纤维可以用作刺激光源，通过照射束中的纤维子集来精确激活不同的神经元模式。通过记录荧光珠在水中的扩散，我们证明了光纤的记录能力。我们的组织学，建模和荧光珠记录表明，光学微纤维可以提供一个微创的，稳定的，双向的接口，用于记录或刺激基因探针在脑深部区域的一种超局部形式的纤维光度。
Optical techniques for recording and manipulating neural activity have traditionally been constrained to superficial brain regions due to light scattering. New techniques are needed to extend optical access to large 3D volumes in deep brain areas, while retaining local connectivity. We have developed a method to implant bundles of hundreds or thousands of optical microfibers, each with a diameter of 8 μm. During insertion, each fiber moves independently, following a path of least resistance. The fibers achieve near total internal reflection, enabling optically interfacing with the tissue near each fiber aperture. At a depth of 3 mm, histology shows fibers consistently splay over 1 mm in diameter throughout the target region. Immunohistochemical staining after chronic implants reveals neurons in close proximity to the fiber tips. Models of photon fluence indicate that fibers can be used as a stimulation light source to precisely activate distinct patterns of neurons by illuminating a subset of fibers in the bundle. By recording fluorescent beads diffusing in water, we demonstrate the recording capability of the fibers. Our histology, modeling and fluorescent bead recordings suggest that the optical microfibers may provide a minimally invasive, stable, bidirectional interface for recording or stimulating genetic probes in deep brain regions—a hyper-localized form of fiber photometry.
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影响因子：
4.6
作者：
Heshmat B;Lee IH;Raskar R
通讯作者：
Raskar R
影响因子：
64.8
作者：
Cui, Guohong;Jun, Sang Beom;Jin, Xin;Pham, Michael D.;Vogel, Steven S.;Lovinger, David M.;Costa, Rui M.
通讯作者：
Costa, Rui M.
DOI：
10.1016/j.ijrobp.2010.06.021
发表时间：
2010-12-01
影响因子：
7
作者：
Bouchet, Audrey;Lemasson, Benjamin;Serduc, Raphael
通讯作者：
Serduc, Raphael
影响因子：
25
作者：
Dombeck, Daniel A.;Harvey, Christopher D.;Tian, Lin;Looger, Loren L.;Tank, David W.
通讯作者：
Tank, David W.
影响因子：
7.8
作者：
Hyvärinen, A;Oja, E
通讯作者：
Oja, E