Functional imaging of hippocampal place cells at cellular resolution during virtual navigation.
Functional imaging of hippocampal place cells at cellular resolution during virtual navigation.
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DOI：
10.1038/nn.2648
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发表时间：
2010-11
影响因子：
25
通讯作者：
Tank, David W.
中科院分区：
文献类型：
--
作者：
Dombeck, Daniel A.;Harvey, Christopher D.;Tian, Lin;Looger, Loren L.;Tank, David W.
空间导航是一种广泛应用于啮齿动物认知、学习和记忆神经回路研究的行为。结合遗传编码指示器的活体显微镜为研究神经元回路提供了重要的新工具，但在导航过程中应用技术困难。我们描述了用亚细胞分辨率成像行为小鼠海马CA1神经元活动的方法。通过慢性海马窗对表达基因编码钙指示物GCaMP3的神经元进行成像。头部固定的小鼠在虚拟现实系统和定制的双光子显微镜相结合的装置中进行空间行为。利用光学方法对位置细胞群进行了识别，并测量了它们在虚拟环境中的位置场位置与其在局部电路中的解剖位置之间的相关性。虚拟现实和高分辨率功能成像的结合应该允许新一代的研究来探测行为过程中的神经元回路动力学。
Spatial navigation is a widely employed behavior in rodent studies of neuronal circuits underlying cognition, learning and memory. In vivo microscopy combined with genetically-encoded indicators provides important new tools to study neuronal circuits, but has been technically difficult to apply during navigation. We describe methods to image the activity of hippocampal CA1 neurons with sub-cellular resolution in behaving mice. Neurons expressing the genetically encoded calcium indicator GCaMP3 were imaged through a chronic hippocampal window. Head-fixed mice performed spatial behaviors within a setup combining a virtual reality system and a custom built two-photon microscope. Populations of place cells were optically identified, and the correlation between the location of their place fields in the virtual environment and their anatomical location in the local circuit was measured. The combination of virtual reality and high-resolution functional imaging should allow for a new generation of studies to probe neuronal circuit dynamics during behavior.
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影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
DOI：
10.1073/pnas.95.6.3182
发表时间：
1998-03-17
影响因子：
11.1
作者：
Fenton, AA;Muller, RU
通讯作者：
Muller, RU
影响因子：
2
作者：
Ergorul, C;Eichenbaum, H
通讯作者：
Eichenbaum, H
影响因子：
2.5
作者：
KANDEL, ER;SPENCER, WA;BRINLEY, FJ
通讯作者：
BRINLEY, FJ
影响因子：
16.2
作者：
Helmchen, F;Fee, MS;Denk, W
通讯作者：
Denk, W