Novel genes participating in the formation of prismatic and nacreous layers in the pearl oyster as revealed by their tissue distribution and RNA interference knockdown.
Novel genes participating in the formation of prismatic and nacreous layers in the pearl oyster as revealed by their tissue distribution and RNA interference knockdown.
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DOI：
10.1371/journal.pone.0084706
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Watabe S
中科院分区：
文献类型：
--
作者：
Funabara D;Ohmori F;Kinoshita S;Koyama H;Mizutani S;Ota A;Osakabe Y;Nagai K;Maeyama K;Okamoto K;Kanoh S;Asakawa S;Watabe S
在我们之前的出版物中，我们通过对珠母贝珍珠贝和棱柱层形成组织的 EST 分析，确定了参与贝壳形成的新候选基因。在本研究中，选择了其中 14 个基因（包括两个已知基因），并使用 RNA 干扰进一步检查了它们在壳形成中的参与情况。基于推导的氨基酸序列的分子表征表明，其中七个新基因编码分泌蛋白。通过RT-PCR和原位杂交分析，基因转录本的组织分布与之前报道的EST分析获得的结果基本一致。注射了靶向基因 000027、000058、000081、000096、000113（珍珠质）、000118、000133 和 000411 (MSI60) 的 dsRNA 的珍珠贝壳，这些基因表现出对珍珠层形成组织的特异性表达，在该层中表现出异常的表面外观。注射了靶向基因 000027、000113 和 000133 的 dsRNA 的个体也表现出异常的棱柱层。注射了靶向基因 000031、000066、000098、000145、000194 和 000200 的 dsRNA 的个体，这些基因显示出对棱柱层形成组织特异的表达，在珠光层和棱柱层中都表现出异常的表面外观。综上所述，结果表明参与棱柱层形成的基因也可能参与珠光层的形成。
In our previous publication, we identified novel gene candidates involved in shell formation by EST analyses of the nacreous and prismatic layer-forming tissues in the pearl oyster Pinctada fucata. In the present study, 14 of those genes, including two known genes, were selected and further examined for their involvement in shell formation using the RNA interference. Molecular characterization based on the deduced amino acid sequences showed that seven of the novel genes encode secretory proteins. The tissue distribution of the transcripts of the genes, as analyzed by RT-PCR and in situ hybridization, was mostly consistent with those obtained by the EST analysis reported previously. Shells in the pearl oysters injected with dsRNAs targeting genes 000027, 000058, 000081, 000096, 000113 (nacrein), 000118, 000133 and 000411 (MSI60), which showed expression specific to the nacreous layer forming tissues, showed abnormal surface appearance in this layer. Individuals injected with dsRNAs targeting genes 000027, 000113 and 000133 also exhibited abnormal prismatic layers. Individuals injected with dsRNAs targeting genes 000031, 000066, 000098, 000145, 000194 and 000200, which showed expression specific to prismatic layer forming tissues, displayed an abnormal surface appearance in both the nacreous and prismatic layers. Taken together, the results suggest that the genes involved in prismatic layer formation might also be involved in the formation of the nacreous layers.
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影响因子：
4.4
作者：
Joubert C;Piquemal D;Marie B;Manchon L;Pierrat F;Zanella-Cléon I;Cochennec-Laureau N;Gueguen Y;Montagnani C
通讯作者：
Montagnani C
影响因子：
3.7
作者：
Fang D;Xu G;Hu Y;Pan C;Xie L;Zhang R
通讯作者：
Zhang R
影响因子：
4.8
作者：
Marin, F;Corstjens, P;Westbroek, P
通讯作者：
Westbroek, P
DOI：
10.1006/bbrc.2000.2274
发表时间：
2000-03-05
影响因子：
3.1
作者：
Kono, M;Hayashi, N;Samata, T
通讯作者：
Samata, T
影响因子：
2.4
作者：
GORDON, J;CARRIKER, MR
通讯作者：
CARRIKER, MR