Identification of genes directly involved in shell formation and their functions in pearl oyster, Pinctada fucata.
Identification of genes directly involved in shell formation and their functions in pearl oyster, Pinctada fucata.
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DOI：
10.1371/journal.pone.0021860
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Fang D;Xu G;Hu Y;Pan C;Xie L;Zhang R
软体动物壳的形成是生物矿化研究的一个有趣的方面。在珍珠贝贝壳形成过程中，贝壳基质蛋白在碳酸钙结晶过程中起着至关重要的作用。描述幼虫发育过程中的生物矿化相关基因有助于加深我们对贝壳形成的理解。通过构建3个抑制消减杂交(SSH)文库来表达幼虫贝壳形成过程中关键点表达的基因，分离了与贝壳生物矿化相关的基因。对这些文库中的2923个EST进行了测序，得到了990个非基因。在3个SSH文库中筛选出分泌蛋白和具有串联重复单位的蛋白的Uniges。获得了一组编码与壳形成有关的基因的序列。对5个基因进行了RT-PCR和原位杂交分析，研究了它们在几种组织中的空间表达，特别是在外套膜组织中的表达。它们都表现出与已知的生物矿化相关基因不同的表达模式。通过RNA干扰抑制这5个基因，导致珍珠层出现不同程度的缺陷，表明它们都参与了文石结晶。耐人寻味的是，有一个基因(UD_Cluster94.seq.Singlet1)被限制在‘文物系’上。据我们所知，目前的数据首次获得了一套在褐藻发育阶段活跃的生物矿化相关基因，其中5个基因负责珍珠层的形成。这为分离与壳形成有关的新基因提供了一个有用的起点。基因对文石线的形成和珍珠层的其他区域的影响表明，文石结晶开始的控制机制与成熟文石生长的控制机制不同。
Mollusk shell formation is a fascinating aspect of biomineralization research. Shell matrix proteins play crucial roles in the control of calcium carbonate crystallization during shell formation in the pearl oyster, Pinctada fucata. Characterization of biomineralization-related genes during larval development could enhance our understanding of shell formation. Genes involved in shell biomineralization were isolated by constructing three suppression subtractive hybridization (SSH) libraries that represented genes expressed at key points during larval shell formation. A total of 2,923 ESTs from these libraries were sequenced and gave 990 unigenes. Unigenes coding for secreted proteins and proteins with tandem-arranged repeat units were screened in the three SSH libraries. A set of sequences coding for genes involved in shell formation was obtained. RT-PCR and in situ hybridization assays were carried out on five genes to investigate their spatial expression in several tissues, especially the mantle tissue. They all showed a different expression pattern from known biomineralization-related genes. Inhibition of the five genes by RNA interference resulted in different defects of the nacreous layer, indicating that they all were involved in aragonite crystallization. Intriguingly, one gene (UD_Cluster94.seq.Singlet1) was restricted to the ‘aragonitic line’. The current data has yielded for the first time, to our knowledge, a suite of biomineralization-related genes active during the developmental stages of P.fucata, five of which were responsible for nacreous layer formation. This provides a useful starting point for isolating new genes involved in shell formation. The effects of genes on the formation of the ‘aragonitic line’, and other areas of the nacreous layer, suggests a different control mechanism for aragonite crystallization initiation from that of mature aragonite growth.
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影响因子：
3.4
作者：
Jackson DJ;Wörheide G;Degnan BM
通讯作者：
Degnan BM
影响因子：
1.1
作者：
Cuif, JP;Dauphin, Y
通讯作者：
Dauphin, Y
DOI：
10.1083/jcb.102.5.1878
发表时间：
1986-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Benson SC;Benson NC;Wilt F
通讯作者：
Wilt F
影响因子：
4.9
作者：
Goldstien, Sharyn J.;Schiel, David R.;Gemmell, Neil J.
通讯作者：
Gemmell, Neil J.
影响因子：
8.6
作者：
Areva, S;Peltola, T;Rosenholm, JB
通讯作者：
Rosenholm, JB