Oxytocin gene expression in discrete hypothalamic magnocellular cell groups is stimulated by prolonged salt loading.
Oxytocin gene expression in discrete hypothalamic magnocellular cell groups is stimulated by prolonged salt loading.
复制标题
长期盐负荷会刺激离散的下丘脑大细胞群中的催产素基因表达。
作者：
H. V. Van Tol;D. Voorhuis;J. Burbach
在大鼠下丘脑不同核团研究了长时间渗透刺激对催产素（OT）产生神经元mRNA水平的影响。饮用2% NaCl 2周后，北方印迹分析显示，视上核（SON）和室旁核（PVN）OT mRNA含量增加2倍。相同的样品显示了类似的变化，血管加压素（VP）mRNA水平。该处理导致脑垂体神经中间叶中OT和VP的70%消耗以及OT和VP血浆水平的显著增加。通过定量原位杂交分析OT mRNA的浓度表明，SON，PVN，和前连合核的反应相似，与1.5倍的增加后，7天的渗透刺激OT mRNA。这些结果表明，OT基因表达的SON，PVN，和前连合核的大细胞神经元是敏感的渗透刺激，是VP基因的SON和PVN的大细胞神经元。OT产生神经元的反应性可能表明OT在调节水和电解质平衡中的作用。
The effect of prolonged osmotic stimulation on the oxytocin (OT) mRNA levels of OT-producing neurons was investigated in separate hypothalamic nuclei of the rat. After drinking 2% NaCl for 2 weeks, a 2-fold increase in the OT mRNA content was found by Northern blot analysis of microdissected supraoptic nucleus (SON) and paraventricular nucleus (PVN). The same samples showed a similar change in vasopressin (VP) mRNA levels. This treatment resulted in a 70% depletion of both OT and VP from the neurointermediate lobe of the pituitary gland and a marked increase in OT and VP plasma levels. Analysis of the OT mRNA concentration by quantitative in situ hybridization showed that the SON, PVN, and anterior commissural nucleus responded similarly, with a 1.5-fold increase in OT mRNA after 7 days of osmotic stimulation. These results demonstrate that OT gene expression in the magnocellular neurons of the SON, PVN, and anterior commissural nucleus is sensitive to this osmotic stimulus, as is the VP gene of magnocellular neurons in the SON and PVN. The responsiveness of OT-producing neurons may point to a role for OT in the regulation of water and electrolyte balance.
影响因子：
4.8
作者：
M. Lumpkin;W. Samson;S. Mccann
通讯作者：
M. Lumpkin;W. Samson;S. Mccann
DOI：
10.1073/pnas.81.3.726
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DESCHENES, RJ;LORENZ, LJ;DIXON, JE
通讯作者：
DIXON, JE
血浆渗透压急剧增加对清醒大鼠血浆加压素相关神经素的影响：对渗透压调节的影响。
影响因子：
4.1
作者：
Cheng,SW;North,WG
通讯作者：
North,WG