Expression and localization of paxillin in rat pancreas during development.
Expression and localization of paxillin in rat pancreas during development.
复制标题
发育过程中大鼠胰腺中桩蛋白的表达和定位。
DOI：
10.3748/wjg.v17.i40.4479
复制
发表时间：
2011
影响因子：
4.3
通讯作者：
W. De
中科院分区：
文献类型：
--
作者：
Jing Guo;Li;Li Yuan;Ning Wang;W. De
目的
研究桩蛋白在大鼠胰腺发育过程中的表达和定位。
方法
本研究使用Sprague道利大鼠胎仔、胚胎、幼龄动物和成年动物的胰腺。采用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blotting）检测桩蛋白在不同发育阶段胰腺中的表达水平。为了确定桩蛋白在发育大鼠胰腺中的细胞位置，分别使用特异性细胞标记物和桩蛋白的抗体进行免疫组织化学和双重免疫荧光染色。
结果
在E15.5（胚胎15.5天）检测到最高的桩蛋白mRNA水平，随后在后期发育阶段降低（分别与E18.5、P0和成体相比，P < 0.05），并且在从E15.5到成体的过渡期间检测到桩蛋白表达逐渐增加。桩蛋白阳性染色主要定位于大鼠胰岛在胰腺发育的各个阶段测试。
结论
桩蛋白在不同发育阶段大鼠胰腺中的动态表达表明桩蛋白可能参与胰腺发育的某些方面。
AIM
To investigate the expression and localization of paxillin in rat pancreas during development.
METHODS
Pancreata from Sprague Dawley rat fetuses, embryos, young animals, and adult animals were used in this study. Expression levels of paxillin in pancreata of different development stages were detected by reverse transcription polymerase chain reaction and Western blotting. To identify the cell location of paxillin in the developing rat pancreas, immunohistochemistry and double-immunofluorescent staining were performed using antibodies for specific cell markers and paxillin, respectively.
RESULTS
The highest paxillin mRNA level was detected at E15.5 (embryo day 15.5) following a decrease in the later developmental periods (P < 0.05 vs E18.5, P0 and adult, respectively), and a progressively increased paxillin protein expression through the transition from E15.5 to adult was detected. The paxillin positive staining was mainly localized in rat islets of Langerhans at each stage tested during pancreas development.
CONCLUSION
The dynamic expression of paxillin in rat pancreas from different stages indicates that paxillin might be involved in some aspects of pancreatic development.
影响因子：
3.3
作者：
Crawford, BD;Henry, CA;Hille, MB
通讯作者：
Hille, MB