Four new type I restriction enzymes identified in Escherichia coli clinical isolates.
Four new type I restriction enzymes identified in Escherichia coli clinical isolates.
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DOI：
10.1093/nar/gni114
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发表时间：
2005-07-21
影响因子：
14.9
通讯作者：
Ryu J
中科院分区：
文献类型：
--
作者：
Kasarjian JK;Kodama Y;Iida M;Matsuda K;Ryu J
采用质粒转化方法和RM搜索计算机程序，从临床分离的大肠杆菌中鉴定出4种具有新识别位点的I型内切酶和2种同分异构体（EcoBI和Eco377I）。这些新酶被命名为Eco394I、Eco826I、Eco851I和Eco912I。它们的识别序列分别为GAC(5N)RTAAY、GCA(6N)CTGA、GTCA(6N)TGAY和CAC(5N)TGGC。甲基化敏感性试验，使用各种合成寡核苷酸，用于鉴定防止切割时甲基化的腺嘌呤（下划线）。这些结果表明I型酶在大肠杆菌和许多其他细菌中丰富，正如从细菌基因组测序项目中推断的那样。
Using a plasmid transformation method and the RM search computer program, four type I restriction enzymes with new recognition sites and two isoschizomers (EcoBI and Eco377I) were identified in a collection of clinical Escherichia coli isolates. These new enzymes were designated Eco394I, Eco826I, Eco851I and Eco912I. Their recognition sequences were determined to be GAC(5N)RTAAY, GCA(6N)CTGA, GTCA(6N)TGAY and CAC(5N)TGGC, respectively. A methylation sensitivity assay, using various synthetic oligonucleotides, was used to identify the adenines that prevent cleavage when methylated (underlined). These results suggest that type I enzymes are abundant in E.coli and many other bacteria, as has been inferred from bacterial genome sequencing projects.
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影响因子：
64.5
作者：
COWAN, GM;GANN, AAF;MURRAY, NE
通讯作者：
MURRAY, NE
影响因子：
5.6
作者：
KANNAN, P;COWAN, GM;MURRAY, NE
通讯作者：
MURRAY, NE
影响因子：
14.9
作者：
Titheradge, AJB;King, J;Murray, NE
通讯作者：
Murray, NE
影响因子：
11.4
作者：
SURI, B;SHEPHERD, JCW;BICKLE, TA
通讯作者：
BICKLE, TA
影响因子：
5.6
作者：
NAGARAJA, V;SHEPHERD, JCW;BICKLE, TA
通讯作者：
BICKLE, TA