The zinc finger of Eco1 enhances its acetyltransferase activity during sister chromatid cohesion.
The zinc finger of Eco1 enhances its acetyltransferase activity during sister chromatid cohesion.
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ECO1的锌指在姐妹染色单体内聚会期间增强其乙酰转移酶活性。
DOI：
10.1093/nar/gkp656
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发表时间：
2009-10
影响因子：
14.9
通讯作者：
Koshland DE
中科院分区：
文献类型：
--
作者：
Onn I;Guacci V;Koshland DE
Eco 1 p/Ctf 7 p是建立姐妹染色单体凝聚所需的一种重要乙酰转移酶。Eco 1 p乙酰化Smc 3 p和Mcd 1 p（Scc 1 p或Rad 21 p），分别在S期和DNA损伤时建立凝聚力。除了乙酰转移酶结构域，Eco 1 p还含有一个保守的锌指结构域。锌指与S期姐妹染色单体凝聚的建立有关，但其在分子水平上的功能及其对损伤诱导凝聚的贡献尚不清楚。在这里，我们表明，锌指是必不可少的建立凝聚力在S期和DNA损伤的反应。我们的研究结果表明，锌指增强乙酰化Eco 1 p本身，Smc 3 p和可能的Mcd 1 p。我们提出，锌指是一个普遍的增强剂的底物识别，从而增强Eco 1 p的能力，乙酰化其基板以上的阈值需要在DNA复制和修复过程中产生凝聚力。最后，我们的锌指的研究发现，Eco 1是一个多聚体，可以利用的属性，以协调乙酰化的底物无论是空间上还是时间上的姐妹染色单体凝聚力的建立。
Eco1p/Ctf7p is an essential acetyltransferase required for the establishment of sister chromatid cohesion. Eco1p acetylates Smc3p and Mcd1p (Scc1p or Rad21p) to establish cohesion during S phase and in response to DNA damage, respectively. In addition to its acetyltransferase domain, Eco1p harbors a conserved zinc finger domain. The zinc finger has been implicated in the establishment of sister chromatid cohesion in S phase, yet its function on the molecular level and its contribution to damage-induced cohesion are unknown. Here, we show that the zinc finger is essential for the establishment of cohesion in both S phase and in response to DNA damage. Our results suggest that the zinc finger augments the acetylation of Eco1p itself, Smc3p and likely Mcd1p. We propose that the zinc finger is a general enhancer of substrate recognition, thereby enhances the ability of Eco1p to acetylate its substrates above a threshold needed to generate cohesion during DNA replication and repair. Finally our studies of the zinc finger led to the discovery that Eco1 is a multimer, a property that could be exploited to coordinate acetylation of substrates either spatially or temporally for establishment of sister chromatid cohesion.
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影响因子：
16
作者：
Haering, CH;Löwe, J;Nasmyth, K
通讯作者：
Nasmyth, K
影响因子：
16
作者：
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影响因子：
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影响因子：
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作者：
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