Comparison of exosomes derived from induced pluripotent stem cells and mesenchymal stem cells as therapeutic nanoparticles for treatment of corneal epithelial defects.
Comparison of exosomes derived from induced pluripotent stem cells and mesenchymal stem cells as therapeutic nanoparticles for treatment of corneal epithelial defects.
复制标题
诱导多能干细胞和间充质干细胞来源的外泌体作为治疗角膜上皮缺陷的治疗性纳米颗粒的比较
DOI：
10.18632/aging.103904
复制
发表时间：
2020-10-13
期刊：
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wang S;Hou Y;Li X;Song Z;Sun B;Li X;Zhang H
诱导多能干细胞和间充质干细胞是多能干细胞，它们在治疗各种组织损伤和伤口愈合方面是很有前景的疗法。外泌体是纳米级的细胞外囊泡，已被确定为治疗功能的重要介质，这些功能是通过细胞通讯实现的。在这项研究中，我们比较了诱导多能干细胞衍生的外泌体（iPSCs - Exos）和间充质干细胞衍生的外泌体（MSCs - Exos）在治疗角膜上皮缺损方面的功效。这两种外泌体的特性没有显著差异。与MSCs - Exos相比，iPSCs - Exos在体外对人角膜上皮细胞的增殖、迁移、细胞周期促进和凋亡抑制具有更好的效果。iPSCs/MSCs - Exos通过上调细胞周期蛋白A和细胞周期蛋白依赖性激酶2（CDK2），促使角膜上皮细胞（HCECs）从G0/G1期进入S期，从而促进细胞再生。角膜上皮缺损模型的体内实验结果表明，iPSCs - Exos和MSCs - Exos都能加速角膜上皮缺损的愈合，而iPSCs - Exos的作用比MSCs - Exos强得多。这项研究表明，iPSCs - Exos对角膜上皮缺损的愈合具有更好的治疗效果。因此，通过使用溶解在滴眼液中的iPSCs - Exos，可以为治疗角膜上皮缺损甚至更多的眼表疾病采取一种新的潜在纳米治疗策略。
Induced pluripotent stem cells and mesenchymal stem cells are pluripotent stem cells that represent promising therapies for treating various tissue injuries and wound healing. Exosomes are nanosized extracellular vesicles that have been identified as important mediators of therapeutic functions, which are performed via cell communication. In this study, we compared the efficacy of induced pluripotent stem cells-derived exosomes (iPSCs-Exos) and mesenchymal stem cells-derived exosomes (MSCs-Exos) in treating corneal epithelial defects. The characteristics of the two types of exosomes were not significantly different. Compared to MSCs-Exos, iPSCs-Exos had a better in vitro effect on the proliferation, migration, cell cycle promotion and apoptosis inhibition of human corneal epithelial cells. iPSCs/MSCs-Exos promoted cell regeneration by upregulating cyclin A and CDK2 to drive HCECs to enter the S phase from the G0/G1 phase. In vivo results from a corneal epithelial defect model showed that both iPSCs-Exos and MSCs-Exos accelerated corneal epithelium defect healing while the effects of iPSCs-Exos were much stronger than those of MSCs-Exos. This study demonstrated that iPSCs-Exos had a better therapeutic effect on corneal epithelial defect healing. Thus, a novel potential nanotherapeutic strategy for treating corneal epithelial defects and even more ocular surface disease could be undertaken by using iPSCs-Exos dissolved in eye drops.
登录
查看更多内容
影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
通讯作者：
Théry C
DOI：
10.1007/10_2012_156
发表时间：
2013-01-01
期刊：
MESENCHYMAL STEM CELLS: BASICS AND CLINICAL APPLICATION II
影响因子：
--
作者：
Eberle, Irina;Moslem, Mohsen;Cantz, Tobias
通讯作者：
Cantz, Tobias
影响因子：
17.8
作者：
Ljubimov AV;Saghizadeh M
通讯作者：
Saghizadeh M
影响因子：
--
作者：
Mort RL;Ramaesh T;Kleinjan DA;Morley SD;West JD
通讯作者：
West JD
磁性水溶性超支化多元醇功能化氧化石墨烯的制备用于高效水体修复
影响因子：
4.6
作者：
Hu L;Li Y;Zhang X;Wang Y;Cui L;Wei Q;Ma H;Yan L;Du B
通讯作者：
Du B