Identification of genes down-regulated during lung cancer progression: a cDNA array study.
Identification of genes down-regulated during lung cancer progression: a cDNA array study.
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DOI：
10.1186/1756-9966-27-38
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发表时间：
2008-09-15
影响因子：
11.3
通讯作者：
Mineo, Tommaso C.
中科院分区：
文献类型：
--
作者：
Campioni, Mara;Ambrogi, Vincenzo;Pompeo, Eugenio;Citro, Gennaro;Castelli, Mauro;Spugnini, Enrico P.;Gatti, Antonio;Cardelli, Pierluigi;Lorenzon, Laura;Baldi, Alfonso;Mineo, Tommaso C.
肺癌仍然是世界上的一大健康挑战。I期疾病患者的存活率在40%至70%之间。这表明，相当大比例的I期非小细胞肺癌患者实际上可能被低估了。为了寻找与肺癌发生相关的基因，我们利用连续的非小细胞肺癌患者(男58例，女6例)进行了基因芯片实验，这些患者的平均年龄为58岁。基本的基因芯片数据确定了14个基因在两组中存在差异表达。定量RT-PCR分析证实，在所分析的14个基因中，有8个基因的转录调控存在有效的差异。这些基因的产物属于不同的功能蛋白类型，如细胞外基质蛋白和蛋白水解酶(Decorin和MMP11)、参与DNA修复的基因(XRCC1)、血管生成调节因子(VEGF)、细胞周期调节因子(Cyclin D1)和肿瘤抑制基因(Semaphorin 3B、WNT-5A和视网膜母细胞瘤相关基因Rb2/p130)。我们的阵列数据证实了之前描述的表达模式的一些差异。此外，我们还首次发现并验证了肺癌进展过程中某些基因表达水平的降低。通过cDNA阵列的比较杂交有助于鉴定一系列新的与进展相关的基因表达变化，同时证实了先前描述的一些结果。
Lung cancer remains a major health challenge in the world. Survival for patients with stage I disease ranges between 40–70%. This suggests that a significant proportion of patients with stage I NSCLC may actually be under-staged. In order to identify genes relevant for lung cancer development, we carried out cDNA array experiments employing 64 consecutive patients (58 men and 6 women) with a median age of 58 years and stage 1 or stage 2 non-small-cell lung cancer (NSCLC). Basic cDNA array data identified 14 genes as differentially regulated in the two groups. Quantitative RT-PCR analysis confirmed an effective different transcriptional regulation of 8 out of 14 genes analyzed. The products of these genes belong to different functional protein types, such as extra-cellular matrix proteins and proteases (Decorin and MMP11), genes involved in DNA repair (XRCC1), regulator of angiogenesis (VEGF), cell cycle regulators (Cyclin D1) and tumor-suppressor genes (Semaphorin 3B, WNT-5A and retinoblastoma-related Rb2/p130). Some previously described differences in expression patterns were confirmed by our array data. In addition, we identified and validated for the first time the reduced expression level of some genes during lung cancer progression. Comparative hybridization by means of cDNA arrays assisted in identifying a series of novel progression-associated changes in gene expression, confirming, at the same time, a number of previously described results.
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DOI：
10.1164/ajrccm.156.6.9612046
发表时间：
1997-12-01
影响因子：
24.7
作者：
Karameris, A;Panagou, P;Bouros, D
通讯作者：
Bouros, D
DOI：
10.1016/s0022-5223(99)70294-1
发表时间：
1999-04-01
影响因子：
6
作者：
D'Amico, TA;Massey, M;Harpole, DH
通讯作者：
Harpole, DH
影响因子：
3.4
作者：
Esposito, V;Baldi, A;Caputi, M
通讯作者：
Caputi, M
影响因子：
11.2
作者：
Kurayoshi, Manabu;Oue, Naohide;Kikuchi, Akira
通讯作者：
Kikuchi, Akira
影响因子：
4.6
作者：
Liu, DG;Huang, CL;Yokomise, H
通讯作者：
Yokomise, H