Co-Electrospun Silk Fibroin and Gelatin Methacryloyl Sheet Seeded with Mesenchymal Stem Cells for Tendon Regeneration.
Co-Electrospun Silk Fibroin and Gelatin Methacryloyl Sheet Seeded with Mesenchymal Stem Cells for Tendon Regeneration.
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DOI：
10.1002/smll.202107714
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发表时间：
2022-05
期刊：
影响因子：
13.3
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Xue, Yumeng;Kim, Han-Jun;Lee, Junmin;Liu, Yaowen;Hoffman, Tyler;Chen, Yi;Zhou, Xingwu;Sun, Wujin;Zhang, Shiming;Cho, Hyun-Jong;Lee, JiYong;Kang, Heemin;Ryu, WonHyoung;Lee, Chang-Moon;Ahadian, Samad;Dokmeci, Mehmet R.;Lei, Bo;Lee, KangJu;Khademhosseini, Ali
丝素蛋白（SF）是一种很有前途的肌腱修复生物材料，但其相对刚性的力学性能和较低的细胞亲和性限制了其在再生医学中的应用。同时，明胶基聚合物在细胞附着和组织重塑方面具有优势，但其机械强度不足以再生肌腱等坚韧组织。考虑到这些方面，在本研究中，明胶甲基丙烯酰（GelMA）与SF结合，创造了一种机械强度高且具有生物活性的纳米纤维支架（SG）。通过改变SF和GelMA的比例，可以灵活地调节SG纳米纤维的力学性能。与SF纳米纤维相比，最佳成分（SG7）的SG纳米纤维上的间充质干细胞（MSCs）表现出更强的生长、增殖、血管内皮生长因子（VEGF）的产生和致腱基因的表达行为。在SG7支架上培养的MSCs的条件培养基中，与单独在SF或GelMA上培养的MSCs相比，纳米纤维可以显著促进细胞的迁移和增殖。组织学分析和肌腱生成相关的免疫荧光染色表明，与其他组相比，SG7支架在体内的肌腱组织再生能力增强。因此，SF和GelMA混合纳米纤维的合理组合可能有助于改善治疗效果，并解决组织工程支架用于肌腱再生的挑战。合理设计的SG复合纤维支架可以通过机械支持MSCs生长，生物诱导MSCs增殖和生长因子分泌，从而显著促进肌腱组织再生过程，在肌腱组织修复中具有广阔的应用前景。
Silk fibroin (SF) is a promising biomaterial for tendon repair, but its relatively rigid mechanical properties and low cell affinity have limited its usefulness and utility in regenerative medicine. Meanwhile, gelatin-based polymers have advantages in cell attachment and tissue remodeling, but have insufficient mechanical strength to regenerate tough tissue such as tendons. Taking these aspects into account, in this study, gelatin methacryloyl (GelMA) was combined with SF to create a mechanically strong and bioactive nanofibrous scaffold (SG). The mechanical properties of SG nanofibers could be flexibly modulated by varying the ratio of SF and GelMA. Compared to SF nanofibers, mesenchymal stem cells (MSCs) seeded on SG fibers with optimal composition (SG7) exhibited enhanced growth, proliferation, vascular endothelial growth factor (VEGF) production and tenogenic gene expression behavior. Conditioned media from MSCs cultured on SG7 scaffolds, compared to MSCs cultured on SF or GelMA alone nanofibers could greatly promote the migration and proliferation of tenocytes. Histological analysis and tenogenesis related immunofluorescence staining indicated SG7 scaffolds demonstrated enhanced in vivo tendon tissue regeneration compared to other groups. Therefore, rational combinations of SF and GelMA hybrid nanofibers may help to improve therapeutic outcomes and address the challenges of tissue-engineered scaffolds for tendon regeneration. The rationally designed SG hybrid fibrous scaffolds could remarkedly promote tendon tissue regeneration process by mechanically support the growth of MSCs and biologically induce the MSCs proliferation and growth factor secretion, enabling their promising application in tendon tissue repair.
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影响因子：
16.1
作者：
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通讯作者：
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