Bionic Silk Fibroin Film Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells by Activating Focal Adhesion Kinase.
Bionic Silk Fibroin Film Promotes Tenogenic Differentiation of Tendon Stem/Progenitor Cells by Activating Focal Adhesion Kinase.
复制标题
DOI：
10.1155/2020/8857380
复制
发表时间：
2020
影响因子：
4.3
通讯作者：
Tang K
中科院分区：
文献类型：
--
作者：
Lu K;Chen X;Tang H;Zhou M;He G;Lu Z;Tang K
肌腱损伤是临床常见的肌肉骨骼系统疾病。由于肌腱再生能力有限，组织工程技术常被用作治疗肌腱损伤的有效方法。丝素蛋白膜具有良好的生物活性和物理性能，适合肌腱再生。本研究旨在制备具有仿生微结构的SF膜，并研究其生物学效应。 制备了具有光滑表面或仿生微结构的SF薄膜。将肌腱干/祖细胞（TSPC）接种于SF膜表面后，检测细胞形态、成腱基因和蛋白表达水平以及粘着斑激酶（FAK）活性，评价SF膜的生物学效应。 具有仿生微结构的SF膜上的TSPCs表现出细长的细胞形态，促进了诸如SCX、TNC、TNMD和COLIA 1等生腱基因和蛋白的表达，并激活了FAK。FAK抑制剂阻断了腱生成基因和蛋白的增强表达。 具有仿生微结构的SF膜可以作为支架，提供生物物理线索以改变细胞粘附排列和细胞形态，并增强TSPC中的tenogenic基因和蛋白质表达。FAK激活在这一生物反应过程中起着关键作用。
Tendon injuries are common musculoskeletal disorders in clinic. Due to the limited regeneration ability of tendons, tissue engineering technology is often used as an effective approach to treat tendon injuries. Silk fibroin (SF) films have excellent biological activities and physical properties, which is suitable for tendon regeneration. The present study is aimed at preparing a SF film with a bionic microstructure and investigating its biological effects. A SF film with a smooth surface or bionic microstructure was prepared. After seeding tendon stem/progenitor cells (TSPCs) on the surface, the cell morphology, the expression level of tenogenic genes and proteins, and the focal adhesion kinase (FAK) activation were measured to evaluate the biological effect of SF films. The TSPCs on SF films with a bionic microstructure exhibited a slender cell morphology, promoted the expression of tenogenic genes and proteins, such as SCX, TNC, TNMD, and COLIA1, and activated FAK. FAK inhibitors blocked the enhanced expression of tenogenic genes and proteins. SF films with a bionic microstructure may serve as a scaffold, provide biophysical cues to alter the cellular adherence arrangement and cell morphology, and enhance the tenogenic gene and protein expression in TSPCs. FAK activation plays a key role during this biological response process.
登录
查看更多内容
DOI：
10.1002/jor.23810
发表时间：
2018-04
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Patel S;Caldwell JM;Doty SB;Levine WN;Rodeo S;Soslowsky LJ;Thomopoulos S;Lu HH
通讯作者：
Lu HH
影响因子：
5.8
作者：
Prabhath A;Vernekar VN;Sanchez E;Laurencin CT
通讯作者：
Laurencin CT
影响因子：
82.9
作者：
Bi, Yanming;Ehirchiou, Driss;Young, Marian F.
通讯作者：
Young, Marian F.
影响因子：
14
作者：
Lawrence BD;Marchant JK;Pindrus MA;Omenetto FG;Kaplan DL
通讯作者：
Kaplan DL
影响因子：
3.8
作者：
Mueller, Sebastian A.;Todorov, Atanas;Majewski, Martin
通讯作者：
Majewski, Martin