Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors.
Comparing Multiplex PCR and Rapid Urease Test in the Detection of H. pylori in Patients on Proton Pump Inhibitors.
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DOI：
10.1155/2012/898276
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发表时间：
2012
影响因子：
2
通讯作者：
Tsang TK
中科院分区：
文献类型：
--
作者：
Chen T;Meng X;Zhang H;Tsang RW;Tsang TK
背景本研究旨在评估多重PCR检测H. pylori感染，并进一步评估有和无PPI治疗患者的CLOtest阴性结果。方法.本研究是一项回顾性队列研究，纳入了457例有消化不良症状的患者，这些患者于2003年6月至2007年10月在埃文斯顿和格伦布鲁克北岸医院接受了上消化道内镜检查。共报告了556份样本，一些患者在此期间进行了一次以上的检测。首先对胃样本进行CLOtest，从该样本中分离DNA并进行一步多重PCR。结果通过M-PCR检测，H.对照组274例中143例（52.2%）检出pylori，PPI组282例中130例（46.1%）检出pylori（P = 0.1746）。CLOtest检测到H的存在。PPI治疗组282例中4例（1.4%）pylori阳性，未治疗组274例中29例（10.6%）pylori阳性（P ≤ 0.0001）。结论我们的PCR灵敏度足以检测到H的存在。pylori，尽管正在接受PPI治疗。
Background. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detect H. pylori infection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at Evanston and Glenbrook Northshore Hospital from June 2003 to October 2007. A total of 556 samples were reported with some patients having more than one test over the time period. The CLOtest was performed first on the gastric specimen and from that specimen, the DNA was isolated and the one-step multiplex PCR was performed. Results. By M-PCR testing, H. pylori was detected in 143 (52.2%) of 274 cases in the control group and 130 (46.1%) of 282 cases in patients on PPI treatment (P = 0.1746). The CLOtest detected the presence of H. pylori in 4 (1.4%) of 282 cases from the same group receiving PPI treatment and 29 (10.6%) of 274 cases from the group not taking a PPI (P ≤ 0.0001). Conclusion. Our PCR is sensitive enough to detect the presence of H. pylori despite being on PPI treatment.
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