Association of a Bacillus species with leaf and twig dieback of Asian pear (Pyrus pyrifolia) in China.

Association of a Bacillus species with leaf and twig dieback of Asian pear (Pyrus pyrifolia) in China.
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芽孢杆菌属与中国亚洲梨(Pyruspyrifolia)叶和枝枯死的关联。

DOI:
10.4454/jpp.v91i3.565
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发表时间:
2009-11
影响因子:
2.2
通讯作者:
--
中科院分区:
农林科学4区
文献类型:
--
作者:

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自2006年以来,在浙江省的两个果园中观察到亚洲梨(Pyrus pyrifolia)叶片和枝条枯梢病。革兰氏阳性菌经常单独分离或与假单胞菌pv.从田间生长的梨的自然感染的枝条中提取的病毒。用这种细菌接种小枝导致叶片和叶柄死亡以及小枝枯死。通过生理生化、碳源利用、脂肪酸甲酯和16S rRNA基因序列分析等多相分析,初步鉴定该菌为芽孢杆菌属(Bacillus pumilus)。这是关于B可能参与的第一份报告。在中国的亚洲梨叶梢枯梢病中,
Leaf and twig dieback of Asian pear (Pyrus pyrifolia) was observed since 2006 in two orchards in Zhejiang Province (China). A gram positive bacterium was frequently isolated alone or in combination with Pseudomonas syringae pv. syringae from naturally infected twigs of field-grown pears. Inoculation of twigs with this bacterium resulted in the death of leaves and petioles as well as in twig dieback. The bacterium was identified as a Bacillus species (possibly Bacillus pumilus) by a polyphasic approach, including physiological and biochemical properties, carbon source utilization profile, fatty acid methyl esters and analysis of 16S rRNA gene sequence. This is the first report of the possible involvement of B. pumilus in leaf and twig dieback of Asian pear in China.
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