Genome-wide identification of WRKY45-regulated genes that mediate benzothiadiazole-induced defense responses in rice.
Genome-wide identification of WRKY45-regulated genes that mediate benzothiadiazole-induced defense responses in rice.
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DOI：
10.1186/1471-2229-13-150
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发表时间：
2013-10-04
影响因子：
5.3
通讯作者：
Takatsuji H
中科院分区：
文献类型：
--
作者：
Nakayama A;Fukushima S;Goto S;Matsushita A;Shimono M;Sugano S;Jiang CJ;Akagi A;Yamazaki M;Inoue H;Takatsuji H
水稻转录因子WRKY 45在水杨酸（SA）/苯并噻二唑（BTH）诱导的抗病性中起着关键作用。它的敲除严重降低了BTH诱导的对真菌病原体稻瘟病菌和细菌病原体黄单胞菌的抗性。水稻（Xoo）。相反，WRKY 45的过表达诱导对这两种病原体的极强抗性。为了阐明WRKY 45依赖的抗病性的分子基础，我们分析了WRKY 45调控的基因表达水稻转化体和瞬时基因表达系统。我们利用WRKY 45-kd基因敲除水稻植株进行了微阵列分析，并在BTH响应基因中鉴定了WRKY 45依赖基因。260个基因的BTH反应性依赖于WRKY 45。其中，220个基因（85%），其中许多编码PR蛋白和蛋白质与次级代谢，上调BTH。这些基因中只有一小部分与OsNPR 1/NH 1调控的基因重叠，支持水稻SA途径分支为WRKY 45调控的和OsNPR 1/NH 1调控的两个亚途径的观点。地塞米松诱导的myc-taged WRKY 45在水稻中的表达立即上调内源WRKY 45和编码转录因子WRKY 62、OsNAC 4和HSF 1的基因的转录，所有这些基因都被报道具有防御相关功能。随后是编码PR蛋白和次级代谢酶的防御基因的上调。这些基因中有许多也是在M.肺部感染。它们的时间转录模式与地塞米松诱导WRKY 45表达后的时间转录模式一致。在瞬时表达系统中，WRKY 45作为效应子反式激活了荧光素酶编码序列与WRKY 62和OsNAC 4的上游和基因内序列融合的报告基因。转录的反式激活通过OsNAC 4上游含有W盒的序列发生，并且W盒中的突变消除了反式激活。这些数据表明WRKY 45在BTH诱导的抗病性中的作用，其作为水稻SA途径中两个分支之一的转录级联调节防御反应的主调节因子。
The rice transcription factor WRKY45 plays a crucial role in salicylic acid (SA)/benzothiadiazole (BTH)-induced disease resistance. Its knockdown severely reduces BTH-induced resistance to the fungal pathogen Magnaporthe oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). Conversely, overexpression of WRKY45 induces extremely strong resistance to both of these pathogens. To elucidate the molecular basis of WRKY45-dependent disease resistance, we analyzed WRKY45-regulated gene expression using rice transformants and a transient gene expression system. We conducted a microarray analysis using WRKY45-knockdown (WRKY45-kd) rice plants, and identified WRKY45-dependent genes among the BTH-responsive genes. The BTH-responsiveness of 260 genes was dependent on WRKY45. Among these, 220 genes (85%), many of which encoded PR proteins and proteins associated with secondary metabolism, were upregulated by BTH. Only a small portion of these genes overlapped with those regulated by OsNPR1/NH1, supporting the idea that the rice SA pathway branches into WRKY45- regulated and OsNPR1/NH1-regulated subpathways. Dexamethazone-induced expression of myc-tagged WRKY45 in rice immediately upregulated transcription of endogenous WRKY45 and genes encoding the transcription factors WRKY62, OsNAC4, and HSF1, all of which have been reported to have defense-related functions. This was followed by upregulation of defense genes encoding PR proteins and secondary metabolic enzymes. Many of these genes were also induced after M. oryzae infection. Their temporal transcription patterns were consistent with those after dexamethazone-induced WRKY45 expression. In a transient expression system consisting of particle bombardment of rice coleoptiles, WRKY45 acted as an effector to trans-activate reporter genes in which the luciferase coding sequence was fused to upstream and intragenic sequences of WRKY62 and OsNAC4. Trans-activation of transcription occurred through a W-box-containing sequence upstream of OsNAC4 and mutations in the W-boxes abolished the trans-activation. These data suggest a role of WRKY45 in BTH-induced disease resistance as a master regulator of the transcriptional cascade regulating defense responses in one of two branches in the rice SA pathway.
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影响因子：
27.5
作者：
Cao, Pei-Jian;Bartley, Laura E.;Ronald, Pamela C.
通讯作者：
Ronald, Pamela C.
影响因子：
11.4
作者：
Eulgem, T;Rushton, PJ;Somssich, IE
通讯作者：
Somssich, IE
影响因子：
7.2
作者：
Friedrich, L;Lawton, K;Ryals, J
通讯作者：
Ryals, J
影响因子：
4.9
作者：
Iwai, Takayoshi;Seo, Shigemi;Ohashi, Yuko
通讯作者：
Ohashi, Yuko
影响因子：
11.6
作者：
CAO, H;BOWLING, SA;DONG, XN
通讯作者：
DONG, XN