Sequential dissection of multiple ionic currents in single cardiac myocytes under action potential-clamp.

Sequential dissection of multiple ionic currents in single cardiac myocytes under action potential-clamp.
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DOI:
10.1016/j.yjmcc.2010.12.020
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发表时间:
2011-03
影响因子:
5
通讯作者:
Chen-Izu Y
Chen-Izu Y
中科院分区:
医学2区
文献类型:
--
作者:
Banyasz T;Horvath B;Jian Z;Izu LT;Chen-Izu Y

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心脏动作电位(AP)是由无数的离子电流形成的。在这项研究中,我们开发了一种创新的AP钳顺序解剖技术,使多个离子电流记录在AP钳下的单细胞。这项新技术比传统的在任何一个电池中只记录一个电流的方法迈出了重要的一步。在单个细胞中测量许多电流的能力揭示了心肌细胞中离子电流的两个迄今为止未知的特征:细胞内电流的协调和细胞间电流的大变化。因此,AP-钳顺序解剖方法为研究单个细胞电生理学提供了独特而强大的工具。
The cardiac action potential (AP) is shaped by myriad ionic currents. In this study we develop an innovative AP-clamp Sequential Dissection technique to enable recording of multiple ionic currents in the single cell under AP-clamp. This new technique presents a significant step beyond the traditional way of recording only one current in any one cell. The ability to measure many currents in a single cell has revealed two hitherto unknown characteristics of the ionic currents in cardiac cells: coordination of currents within a cell and large variation of currents between cells. Hence, the AP-clamp Sequential Dissection method provides a unique and powerful tool for studying individual cell electrophysiology.
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