Effect of the standard herbal preparation, STW5, treatment on dysbiosis induced by dextran sodium sulfate in experimental colitis.
Effect of the standard herbal preparation, STW5, treatment on dysbiosis induced by dextran sodium sulfate in experimental colitis.
复制标题
标准中药制剂stw5对葡聚糖硫酸钠诱导的实验性结肠炎生物失调的治疗作用。
DOI：
10.1186/s12906-021-03337-8
复制
发表时间：
2021-06-08
影响因子：
3.9
通讯作者：
Khayyal MT
中科院分区：
文献类型：
--
作者：
Mohamed SS;Abdeltawab NF;Wadie W;Ahmed LA;Ammar RM;Rabini S;Abdel-Aziz H;Khayyal MT
标准化的草药制剂STW 5在功能性胃肠道疾病中临床有效，在溃疡性结肠炎（UC）中实验有效。本研究探讨了STW 5的有益作用是否涉及影响肠道微生物群。Wistar大鼠通过饮用含5%葡聚糖硫酸钠（DSS）的水连续7天诱发UC。同时用STW 5处理大鼠，并在末次给药后24 h处死。粪便样品用于使用实时PCR确定所选微生物门和属的丰度的变化。UC的诱导导致肠道微生物群的生态失调和变化。这些变化包括厚壁菌门的一些属，即肠球菌属的增加，以及其他属，即布劳特氏菌属、梭菌属和乳杆菌属的减少。DSS进一步诱导拟杆菌和变形菌的丰度以及放线菌及其属双歧杆菌的相对丰度显著增加。甲烷短杆菌（宽古菌门）的水平也有所增加。微生物生态失调与结肠炎症的各种参数的变化相关。STW 5有效地防止了这些变化，并显著影响UC模型中的水肿和炎症指数。结肠长度的变化，结肠质量指数，炎症和凋亡标志物，和DSS诱导的组织学变化也被阻止。菌群失调在DSS诱导的UC的发展中起着促进作用。微生物植物群的紊乱和相关的炎症过程在很大程度上被STW 5阻止，这表明这种作用可能有助于其在这种情况下的有益用途。在线版本包含补充材料，可通过10.1186/s12906-021-03337-8获得。
The standardized herbal preparation, STW 5, is effective clinically in functional gastrointestinal disorders and experimentally in ulcerative colitis (UC). The present study explores whether the beneficial effect of STW 5 involves influencing the intestinal microbiota. UC was induced in Wistar rats by feeding them 5% dextran sodium sulfate (DSS) in drinking water for 7 days. Rats were treated concurrently with STW 5 and sacrificed 24 h after last drug administration. Fecal samples were used to determine changes in the abundance of selected microbial phyla and genera using real-time PCR. Induction of UC led to dysbiosis and changes in the gut microbiota. The changes included an increase in some genera of the Firmicutes, namely Enterococcus, and a decrease in others, namely Blautia, Clostridium, and Lactobacillus. DSS further induced a marked increase in the abundance of Bacteroidetes and Proteobacteria as well as in the relative abundance of Actinobacteria and its genus Bifidobacterium. Methanobrevibacter levels (phylum Euryarchaeota) were also increased. Microbial dysbiosis was associated with changes in various parameters of colonic inflammation. STW 5 effectively guarded against those changes and significantly affected the indices of edema and inflammation in the UC model. Changes in colon length, colon mass index, inflammatory and apoptotic markers, and histological changes induced by DSS were also prevented. Dysbiosis plays a contributing role in the development of DSS-induced UC. Derangements in the microbial flora and associated inflammatory processes were largely prevented by STW 5, suggesting that this effect might contribute towards its beneficial usefulness in this condition. The online version contains supplementary material available at 10.1186/s12906-021-03337-8.
登录
查看更多内容
影响因子：
3.1
作者：
Munyaka, Peris Mumbi;Rabbi, Mohammad Fazle;Ghia, Jean-Eric
通讯作者：
Ghia, Jean-Eric
影响因子：
3.7
作者：
Laroui H;Ingersoll SA;Liu HC;Baker MT;Ayyadurai S;Charania MA;Laroui F;Yan Y;Sitaraman SV;Merlin D
通讯作者：
Merlin D
影响因子：
0.9
作者：
Ottillinger, Bertram;Storr, Martin;Allescher, Hans-Dieter
通讯作者：
Allescher, Hans-Dieter
DOI：
10.1016/j.ijfoodmicro.2010.12.020
发表时间：
2011-09-01
影响因子：
5.4
作者：
Philippe, David;Heupel, Esther;Riedel, Christian U.
通讯作者：
Riedel, Christian U.
影响因子：
7.9
作者：
Abdel-Aziz, Heba;Wadie, Walaa;Khayyal, Mohamed T.
通讯作者：
Khayyal, Mohamed T.