Rumen Bacteria Communities and Performances of Fattening Lambs with a Lower or Greater Subacute Ruminal Acidosis Risk.
Rumen Bacteria Communities and Performances of Fattening Lambs with a Lower or Greater Subacute Ruminal Acidosis Risk.
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亚急性瘤胃酸中毒风险较低或较高的育肥羔羊的瘤胃细菌群落和表现
DOI：
10.3389/fmicb.2017.02506
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发表时间：
2017
影响因子：
5.2
通讯作者：
Weng X
中科院分区：
文献类型：
--
作者：
Li F;Wang Z;Dong C;Li F;Wang W;Yuan Z;Mo F;Weng X
几种瘤胃纤维素分解菌对pH敏感，因此可用作生物标志物来确定育肥羔羊亚急性瘤胃酸中毒（SARA）的风险。本研究比较了饲喂后2-4 h的瘤胃pH测量值与瘤胃pH敏感细菌的丰度，以评估120只肥育羔羊中SARA的风险。羔羊在单个单元中饲养50天。在第51天饲喂后2-4 h，通过使用口胃管收集瘤胃液。虽然羔羊饲喂相同的饮食，他们的反应不同的丰度瘤胃pH敏感的纤维素分解细菌（瘤胃球菌白，瘤胃球菌flavefaciens和Fibrobacter succinogenes和Butyrivibrio fibrisolvens）。然后将具有最多或最少纤维素分解细菌的羔羊分别分类为较低SARA风险组（LSR，n = 10）或较高SARA风险组（HSR，n = 10）。结果表明，瘤胃pH值和VFA与纤维分解菌数量无显著相关性（P > 0.050）。HSR组羔羊瘤胃pH值（P = 0.013）和乙酸/丙酸比值（P = 0.018）低于LSR组，乳酸浓度（P = 0.035）和丙酸比例（P = 0.033）高于LSR组。两种羔羊的日增重和日增重差异不显著（P > 0.050）。多样性分析显示，显着较低的多样性HSR羔羊比LSR（辛普森指数，P = 0.004）。拟杆菌门、纤维杆菌门、疣微菌门和变形菌门的相对丰度在LSR羔羊中显著高于HSR羔羊（P < 0.050）。其中厚壁菌门（Firmicutes）、韧壁菌门（Tenericutes）和放线菌门（Actinobacteria）的丰度在HSR组高于LSR组（P < 0.050）。根据Unifrac距离，LSR和HSR的瘤胃细菌群落分别聚类，表明LSR和HSR羔羊之间在OTU水平上的细菌群落不同。总体而言，羔羊瘤胃pH值与pH敏感细菌的丰度之间无相关性，pH敏感细菌的数量可作为羔羊SARA的潜在生物标志物。
Several ruminal cellulolytic bacteria species are sensitive to pH and could therefore be used as biomarkers to determine the risk of sub-acute ruminal acidosis (SARA) in finishing lambs. This study compared a 2–4 h post feeding ruminal pH measurement to abundances of the ruminal pH-sensitive bacteria to evaluate the risk of SARA in a herd of 120 finishing lambs. The lambs were reared in individual units for 50 days. Ruminal fluid was collected by use of an orogastric tube on day 51 2-4 h after feeding. Although the lambs were fed an identical diet, they responded differently in the abundances of four ruminal pH sensitive cellulolytic bacteria (Ruminococcus albus, Ruminococcus flavefaciens and Fibrobacter succinogenes and Butyrivibrio fibrisolvens). Lambs with the most or the least cellulolytic bacteria were then classified as either lower SARA risk (LSR, n = 10) or higher SARA risk group (HSR, n = 10), respectively. Data showed that the ruminal pH and VFA profiles were uncorrelated with the number of cellulolytic bacteria (P > 0.050). Lambs with the HSR showed lower ruminal pH (P = 0.013) and acetate to propionate ratio (P = 0.018), higher concentrations of lactate (P = 0.035) and proportion of propionate (P = 0.033) compared to those with the LSR. The DMI and ADG did not differ in LSR and HSR lambs (P > 0.050). A diversity analysis revealed significantly lower diversity in HSR lambs than in LSR (Simpson index, P = 0.004). The relative abundances of the phyla Bacteroidetes, Fibrobacteres, Verruomicrobia, and Proteobacteria were higher in LSR lambs than in HSR (P < 0.050). The abundances of several phyla including Firmicutes, Tenericutes and Actinobacteria were higher in the HSR than in the LSR group (P < 0.050). The bacterial communities of the LSR and HSR clustered separately in rumen based on the Unifrac distances, indicating distinct bacteria communities at OTU level between the LSR and HSR lambs. Overall, there was no correlation between 2 and 4 h post-feeding ruminal pH and the abundance of pH-sensitive bacteria and the amount of these bacteria could be used as a potential biomarker of SARA in lamb herd.
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影响因子：
2.3
作者：
Mickdam, Elsayed;Khiaosa-ard, Ratchaneewan;Zebeli, Qendrim
通讯作者：
Zebeli, Qendrim
影响因子：
3.5
作者：
Mohammed, R.;Stevenson, D. M.;Beauchemin, K. A.
通讯作者：
Beauchemin, K. A.
影响因子：
5.2
作者：
Li F;Henderson G;Sun X;Cox F;Janssen PH;Guan le L
通讯作者：
Guan le L
影响因子：
3.5
作者：
Gao, X.;Oba, M.
通讯作者：
Oba, M.
影响因子：
4.4
作者：
Fernando, S. C.;Purvis, H. T., II;DeSilva, U.
通讯作者：
DeSilva, U.