5-Lipoxygenase DNA methylation and mRNA content in the brain and heart of young and old mice.
5-Lipoxygenase DNA methylation and mRNA content in the brain and heart of young and old mice.
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DOI：
10.1155/2009/209596
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发表时间：
2009
影响因子：
3.1
通讯作者：
Manev H
中科院分区：
文献类型：
--
作者：
Dzitoyeva S;Imbesi M;Ng LW;Manev H
The expression of 5-lipoxygenase (5-LOX) is affected by aging and regulated by epigenetic mechanisms including DNA methylation. We used methylation-sensitive restriction endonucleases (AciI, BstUI, HpaII, and HinP1I) to assess 5-LOX DNA methylation in brain and heart tissue samples from young (2 months) and old (22 months) mice. We also measured mRNA content for 5-LOX and the DNA methyltransferases DNMT1 and DNMT3a. In young mice, the 5-LOX mRNA content was significantly greater in the heart compared to the brain; 5-LOX DNA methylation was lower, except in the AciI assay in which it was higher in the heart. Aging decreased 5-LOX mRNA content in the heart and increased it in the brain. Aging also increased 5-LOX DNA methylation and this effect was site- (i.e., enzyme) and tissue-specific. Generally, DNMT1 and DNMT3a mRNA content was lower in the brain regions compared to the heart; the only effect of aging was observed in the mRNA content of DNMT3a, which was decreased in the heart of old mice. These results indicate a complex tissue-specific and aging-dependent interplay between the DNA methylation system and 5-LOX mRNA content. Interpretation of this data must take into account that the tissue samples contained a mixture of various cell types.
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DOI：
10.1073/pnas.0607521104
发表时间：
2007-01-02
影响因子：
11.1
作者：
Oakes, C. C.;La Salle, S.;Trasler, J. M.
通讯作者：
Trasler, J. M.
DOI：
10.1111/j.1748-1716.1979.tb06449.x
发表时间：
1979-01-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
KAINULAINEN, H;PILSTROM, L;VIHKO, V
通讯作者：
VIHKO, V
影响因子：
4.7
作者：
Farias, Santiago E.;Zarini, Simona;Heidenreich, Kim A.
通讯作者：
Heidenreich, Kim A.
影响因子：
4.2
作者：
Chinnici, Cinzia M.;Yao, Yuemang;Pratico, Domenico
通讯作者：
Pratico, Domenico
影响因子：
3.5
作者：
Ménard, C;Valastro, B;Massicotte, G
通讯作者：
Massicotte, G