3D Cell Culture—Can It Be As Popular as 2D Cell Culture?
3D Cell Culture—Can It Be As Popular as 2D Cell Culture?
复制标题
3D 细胞培养——它能像 2D 细胞培养一样受欢迎吗？
DOI：
10.1002/anbr.202000066
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发表时间：
2021-02
影响因子：
3.4
通讯作者：
Yong He
中科院分区：
文献类型：
--
作者：
Miao Sun;An Liu;Xiaofu Yang;Jiaxing Gong;Mengfei Yu;Xinhua Yao;Huiming Wang;Yong He
A 2D cell culture, a technology used for a long time in the field of life sciences, refers to the growth of cells on a flat surface. The application of this technology enables humans to study the physiological and pathological activities of cells in vitro. However, with the gradual understanding of the concept of cell microenvironment, scientists have discovered that the physiological state and activity of cells in the growth environment of 2D culture are not completely consistent with the cells in vivo. The results of 2D cell experiments often contradict those obtained from animal experiments and clinical experiments. Therefore, in the past ten years, scientists have devoted themselves to the development of various 3D culture techniques to provide cells with a culture environment that more closely resembles that of in vivo milieu. To date, scientists have performed 3D cell culture by changing the culture matrix. Initially, some researchers used culture plates with ultralow attachment (ULA) surfaces to culture tumor spheres. This kind of culture plate has a covalently bound hydrogel layer on the surface to prevent cells from adhering to the culture plate. The microplate, designed with a spherical bottom, allows the cells to clump together to form small spheres (diameter< 1000 μm). On such a microplate, two or even three kinds of cells can be cultured individually or co-cultured with each other. The cell sphere, thus, formed is regarded as tissue units or organoids, which can be used for drug screening research or organ development research. For example, studies have demonstrated that breast cancer cells grown as agglomerates are more resistant to paclitaxel and doxorubicin than those grown in 2D culture. It is suggested that the formation of 3D structures protects tumor cells from drug-induced apoptosis induced by drug. Compared with the mouse primary hepatocyte model, the mouse liver microsphere tissue model cultured using this method exhibited a response to indocyanine green drug-induced damage, which more closely resembles that of the mouse liver model. In a culture of 3D cell spheres, the stereoscopic cells showed a physiological function resembling those naturally present in body. However, due to the lack of an effective nutrient supply system, necrosis often occurs in the center of the cell sphere in the later period of the culture or when the volume of the cell sphere is large. Natural cells grow in an extracellular matrix (ECM) system composed of collagen fibers, glycosaminoglycans, Dr. M. Sun, Dr. X. Yang, Dr. J. Gong, Dr. M. Yu, Prof. H. Wang The Affiliated Hospital of Stomatology School of Stomatology Zhejiang University School of Medicine and Key Laboratory of Oral Biomedical Research of Zhejiang Province Hangzhou, Zhejiang 310000, China E-mail: whmwhm@zju.edu.cn Dr. A. Liu Department of Orthopaedic Surgery Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310000, China Dr. X. Yao, Prof. Y. He Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province School of Mechanical Engineering Zhejiang University Hangzhou 310000, China E-mail: yongqin@zju.edu.cn Prof. Y. He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical Engineering Zhejiang University Hangzhou 310000, China
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影响因子：
9.7
作者：
Zhao, Xinxin;Irvine, Scott Alexander;Venkatraman, Subbu S.
通讯作者：
Venkatraman, Subbu S.
DOI：
10.1016/j.jconrel.2020.04.039
发表时间：
2020-04
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
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影响因子：
4.6
作者：
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通讯作者：
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影响因子：
3.4
作者：
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通讯作者：
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影响因子：
9
作者：
Lv, Shang;Nie, Jing;He, Yong
通讯作者：
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