Induction of cardiac myogenic lineage development differs between mesenchymal and satellite cells and is accelerated by bone morphogenetic protein-4.
Induction of cardiac myogenic lineage development differs between mesenchymal and satellite cells and is accelerated by bone morphogenetic protein-4.
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DOI：
10.1016/j.yjmcc.2012.06.003
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发表时间：
2012-09
影响因子：
5
通讯作者：
Geenen DL
中科院分区：
文献类型：
--
作者：
Grajales L;García J;Geenen DL
Our aim was to further elucidate the cardiac lineage development of bone marrow-derived mesenchymal stem cells (MSC) and to identify cells which had the potential for cardiac myogenic differentiation when compared to skeletal muscle satellite (Sk-sat) myogenesis. Unlike Sk-sat, MSC expressed the early cardiac markers Nkx2.5 and GATA4. Their expression was significantly increased by culturing MSC with Bone Morphogenetic Protein 4 (BMP4). Enhanced cardiac myogenic lineage differentiation and loss of stem cell characteristics induced by BMP4 were further confirmed by flow cytometry of cells stained for Nkx2.5 and Sca-1 expression. MSC also expressed skeletal genes (MyoG, ssTnI, Sk-Act) early in culture but their expression was suppressed when BMP4 was added from day 0–6 (p < 0.05). BMP4 treated MSC also exhibited a 6-fold increase in cTnI expression by day 12 in culture. The average MSC action potential time duration at 90% (APD90) was 32.3 ± 4 ms, with some cells exhibiting action potentials closer to Sk-sat APD90 of 13.7 ± 0.9 ms. After treatment with BMP4, MSC significantly increased their APD90 to 54.4 ± 7.6 ms, shifting from the shorter skeletal-like signature, towards a longer action potential duration more characteristic of a cardiomyocyte signature. Our results show that MSC and Sk-sat exhibit similarities in myogenic lineage development early in culture but that BMP4 clearly enhances cardiac myogenic development, suppresses skeletal myogenesis, and leads to loss of “stemness” in MSC. These findings provide novel information regarding the use of BMP4 to accelerate cardiac myogenic development in harvested MSC and further support the use of MSC in cardiac regenerative therapy.
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DOI：
10.1111/j.1527-5299.2002.00292.x
发表时间：
2002-07-01
期刊：
Congestive heart failure (Greenwich, Conn.)
影响因子：
--
作者：
Muller-Ehmsen, Jochen;Kedes, Laurence H;Kloner, Robert A
通讯作者：
Kloner, Robert A
影响因子：
12.4
作者：
Xiang, Guosheng;Yang, Qing;Huard, Johnny
通讯作者：
Huard, Johnny
影响因子：
5.2
作者：
Gepstein, Lior;Ding, Chunhua;Olgin, Jeffery E.
通讯作者：
Olgin, Jeffery E.
影响因子：
64.8
作者：
Yang, Lei;Soonpaa, Mark H.;Keller, Gordon M.
通讯作者：
Keller, Gordon M.
影响因子：
2.2
作者：
Hare JM;Chaparro SV
通讯作者：
Chaparro SV