Sertoli Cell Vacuolization and Abnormal Germ Cell Adhesion in Mice Deficient in an Inositol Polyphosphate 5-Phosphatase1
Sertoli Cell Vacuolization and Abnormal Germ Cell Adhesion in Mice Deficient in an Inositol Polyphosphate 5-Phosphatase1
复制标题
肌醇多磷酸 5-磷酸酶缺陷小鼠中支持细胞空泡化和生殖细胞粘附异常1
作者：
E. Hellsten;D. Bernard;J. Owens;M. Eckhaus;S. Suchy;R. Nussbaum
Abstract The dynamic nature of cellular interactions during differentiation of germ cells and their translocation from the basement membrane to the lumen of the seminiferous tubules requires the existence of complex and well-regulated cellular adhesion mechanisms in the testis. Successful migration of the developing germ cells is characterized by dynamic breakage and reformation of cadherin-containing adherens junctions between the germ cells and Sertoli cells, the polarized somatic cells of the testis that support and nourish the developing gametes. Here, we demonstrate the accumulation of abnormally swollen, actin-coated, endosome-like structures that contain intact adherens junctions and stain positive for N-cadherin and β-catenin in the Sertoli cell cytosol of mice deficient in Inpp5b, an inositol polyphosphate 5-phosphatase. Simultaneous to the formation of these abnormal structures, developing germ cells are prematurely released from the seminiferous epithelium and sloughed into the epididymis. Our results demonstrate a role for Inpp5b in the regulation of cell adhesion in the testis and in the formation of junctional complexes with neighboring cells, and they emphasize the important and essential role of phosphoinositides in spermatogenesis.
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影响因子：
3.6
作者：
SYLVESTER, SR;MORALES, C;GRISWOLD, MD
通讯作者：
GRISWOLD, MD
影响因子：
4.1
作者：
EBERHARD, DA;COOPER, CL;HOLZ, RW
通讯作者：
HOLZ, RW
大鼠睾丸中转铁蛋白和转铁蛋白受体的定位。
影响因子：
3.6
作者：
Sylvester,SR;Griswold,MD
通讯作者：
Griswold,MD
影响因子：
2.7
作者：
Hellsten, E;Evans, JP;Nussbaum, RL
通讯作者：
Nussbaum, RL