Transcranial flavoprotein-autofluorescence imaging of sound-evoked responses in the mouse auditory cortex under three types of anesthesia
Transcranial flavoprotein-autofluorescence imaging of sound-evoked responses in the mouse auditory cortex under three types of anesthesia
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三种麻醉下小鼠听觉皮层声音诱发反应的经颅黄素蛋白自体荧光成像
DOI：
10.1016/j.neulet.2016.09.021
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发表时间：
2016
影响因子：
2.5
通讯作者：
and T. Tateno
中科院分区：
文献类型：
--
作者：
Y. Yanagawa;H. Osanai;and T. Tateno
麻醉对皮层神经元功能听觉特性的影响，如空间和时间反应特性，在麻醉和清醒受试者之间是不同的。然而，研究表明，接近清醒状态的合适的麻醉方法仍然有用，因为它更稳定和可控性更强。本研究比较了三种麻醉条件下小鼠听皮层两个核心区的神经反应特性：乌拉坦；氯胺酮和盐酸赛拉津(KX)混合物；以及美多咪定、咪达唑仑和布托啡诺(MMB)的组合。为了测量体内的声音刺激，我们记录了内源性绿色荧光的黄素蛋白自体荧光图像。在所有条件下，观察听觉核心区对单音反应的荧光变化，并分析反应特性，如峰值强度、潜伏期、持续时间和激活区域。结果表明，在氨基甲酸乙酯作用下，核心区的反应峰强度、潜伏期和持续时间均大于KX和MMB，而KX和MMB之间无显著差异。相反，在KX麻醉下，激活区以一种子场依赖的方式表现出特有的反应特性。这些结果显示了麻醉对听觉皮质核心区反应特性的不同影响。
The effects of anesthesia on the functional auditory characteristics of cortical neurons, such as spatial and temporal response properties, vary between an anesthetized and an awake subject. However, studies have shown that an appropriate anesthetic method that approaches the awake condition is still useful because of its greater stability and controllability. The present study compared neural response properties from two core fields of the mouse auditory cortex under three anesthetic conditions: urethane; ketamine and xylazine hydrochloride (KX) mixture; and a combination of medetomidine, midazolam, and butorphanol (MMB). To measure sound stimulationin vivo, we recorded flavoprotein-autofluorescent images of endogenous green fluorescence. Under all conditions, fluorescence changes in auditory core subfields in response to tones were observed, and response properties, such as peak intensity, latency, duration, and activated areas were analyzed. Results showed larger response peak intensity, latency, and duration in the core subfields under urethane compared with KX and MMB, with no significant differences between KX and MMB. Conversely, under KX anesthesia the activated areas showed characteristic response properties in a subfield-dependent manner. These results demonstrated the varied effects of anesthesia on response properties in the core subfields of the auditory cortex.
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注射麻醉药的药理学
DOI：
--
发表时间：
1997
期刊：
影响因子：
--
作者：
R. Fish
通讯作者：
R. Fish
受基础条件和麻醉类型影响，心血管对外周给药 GABA 的反应存在差异
影响因子：
7.3
作者：
S. Giuliani;C. Maggi;A. Meli
通讯作者：
A. Meli
美托咪定——一种新型α2-肾上腺素受体激动剂：药效学作用综述
DOI：
10.1016/0278-5846(89)90051-1
发表时间：
1989
影响因子：
5.6
作者：
H. Scheinin;R. Virtanen;E. Macdonald;R. Lammintausta;M. Scheinin
通讯作者：
M. Scheinin
氯胺酮麻醉与美托咪定、咪达唑仑和布托啡诺混合物麻醉下大鼠初级听觉皮层的神经反应差异
影响因子：
2.8
作者：
Y. Yanagawa;H. Osanai;and T. Tateno;H. Osanai and T. Tateno
通讯作者：
H. Osanai and T. Tateno
Sax工业材料的危险特性：1993年更新
DOI：
--
发表时间：
1994
期刊：
--
影响因子：
--
作者：
Richard J. Lewis;N. Sax
通讯作者：
N. Sax