Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules.
Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules.
复制标题
基于纸张的荧光RNA适体传感器，用于无标记的小分子。
DOI：
10.1039/d0ay00588f
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发表时间：
2020-06-04
期刊：
影响因子：
--
通讯作者：
You M
中科院分区：
文献类型：
--
作者：
Shafiei F;McAuliffe K;Bagheri Y;Sun Z;Yu Q;Wu R;You M
近年来出现了基于荧光RNA适体的传感器。这些传感器已被用于在体外和细胞内检测范围广泛的生物和医学目标。然而，基于荧光RNA的传感器用于即时检测的潜在应用仍然很少研究。在这里，我们报告了一个基于纸基板的便携式荧光RNA传感器系统。目标检测可以简单地通过RNA传感器嵌入滤纸的再水化进行。这种价格实惠的传感器系统可用于选择性，灵敏和快速检测不同的目标分析物，如抗生素和细胞信号分子。我们相信，这些纸基荧光RNA传感器在从小分子、核酸、蛋白质到各种病原体的广泛目标的即时检测中表现出巨大的潜力。一种纸基便携式荧光RNA传感器，用于目标分析物的选择性、灵敏性和快速检测。
Sensors based on fluorogenic RNA aptamers have emerged in recent years. These sensors have been used for in vitro and intracellular detection of a broad range of biological and medical targets. However, the potential application of fluorogenic RNA-based sensors for point-of-care testing is still little studied. Here, we report a paper substrate-based portable fluorogenic RNA sensor system. Target detection can be simply performed by rehydration of RNA sensor-embedded filter papers. This affordable sensor system can be used for the selective, sensitive, and rapid detection of different target analytes, such as antibiotics and cellular signaling molecules. We believe these paper-based fluorogenic RNA sensors exhibit a great potential for point-of-care testing of a wide range of target from small molecules, nucleic acids, proteins, to various pathogens. A paper-based portable fluorogenic RNA sensor for the selective, sensitive, and rapid detection of target analytes.
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DOI：
10.1126/science.1207339
发表时间：
2011-07-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Paige JS;Wu KY;Jaffrey SR
通讯作者：
Jaffrey SR
影响因子：
64.5
作者：
Pardee K;Green AA;Ferrante T;Cameron DE;DaleyKeyser A;Yin P;Collins JJ
通讯作者：
Collins JJ
DOI：
10.1073/pnas.1720406115
发表时间：
2018-06-05
影响因子：
11.1
作者：
Sherlock ME;Sudarsan N;Breaker RR
通讯作者：
Breaker RR
影响因子：
15
作者：
Karunanayake Mudiyanselage APKK;Yu Q;Leon-Duque MA;Zhao B;Wu R;You M
通讯作者：
You M
DOI：
10.3390/molecules23040795
发表时间：
2018-03-30
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Manyi-Loh C;Mamphweli S;Meyer E;Okoh A
通讯作者：
Okoh A