Synthesis of Heat Shock/Stress Proteins during Cellular Injury
Synthesis of Heat Shock/Stress Proteins during Cellular Injury
复制标题
细胞损伤期间热休克/应激蛋白的合成
DOI：
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发表时间：
1993
影响因子：
5.2
通讯作者：
T. Nowak
中科院分区：
文献类型：
--
作者：
T. Nowak
从大脑中应激蛋白表达的可用数据中可以得出几个结论，它们是细胞损伤的标记物。首先，很明显，大脑中的所有细胞类型都能够表达应激蛋白，尽管对特定侮辱的反应在人群中存在显著的特异性。在给定的模型中，hsp72表达所显示的明显的反应性等级与特定细胞群的相对易感性密切相关。随着损伤严重程度的增加，应激反应的翻译成分可能会减弱，因为即使hsp72的mRNA大量表达，其免疫反应性也无法积累。出于这个原因，hsp72免疫反应性提供了一个细胞群的指数，这些细胞群对侮辱做出了功能性应激反应。这种反应并不足以保证存活，因为在大鼠全身缺血后，许多显示显著hsp72染色的CA1神经元最终会丢失。然而，导致hsp72和其他由诱导mrna编码的蛋白质表达的短暂损伤确实导致对后续损伤的耐受。未来的研究有望揭示特定基因产物对耐受性状态的贡献。同时，hsp72 mRNA和蛋白表达的互补评估提供了鉴定对不同损伤有反应的细胞群的实用手段。
Several conclusions can be drawn from available data on the expression of stress proteins in brain with respect to their utility as markers of cellular injury. First, it is evident that all cell types in brain are capable of expressing stress proteins, although there is striking specificity in the population responding to a given insult. The apparent hierarchy of responsiveness indicated by hsp72 expression correlates well with the relative vulnerability of specific cell populations in a given model. With increasing severity of injury there can be an attenuation of the translational component of the stress response, in that hsp72 immunoreactivity fails to accumulate even though its mRNA is abundantly expressed. For this reason, hsp72 immunoreactivity provides an index of cell populations that have responded to an insult with a functional stress response. Such a response is not sufficient to guarantee survival, since many CA1 neurons that show significant hsp72 staining are eventually lost after global ischemia in the rat. However, brief insults that result in expression of hsp72 and other proteins encoded by induced mRNAs do result in tolerance to subsequent insults. Future studies may be expected to reveal the contributions of specific gene products to the tolerant state. Meanwhile, complementary evaluations of hsp72 mRNA and protein expression provide practical means of identifying cell populations responding to diverse injuries.
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影响因子：
56.9
作者：
FLYNN, GC;CHAPPELL, TG;ROTHMAN, JE
通讯作者：
ROTHMAN, JE
DOI：
10.1073/pnas.88.16.6911
发表时间：
1991-08-01
影响因子：
11.1
作者：
SCHUETZ, TJ;GALLO, GJ;KINGSTON, RE
通讯作者：
KINGSTON, RE
哺乳动物 70,000 道尔顿应激蛋白的快速纯化：蛋白质对核苷酸的亲和力。
影响因子：
5.3
作者：
Welch,WJ;Feramisco,JR
通讯作者：
Feramisco,JR
影响因子：
56.9
作者：
BECKMANN, RP;MIZZEN, LA;WELCH, WJ
通讯作者：
WELCH, WJ
DOI：
10.1073/pnas.87.16.6263
发表时间：
1990-08-01
影响因子：
11.1
作者：
BENJAMIN, IJ;KROGER, B;WILLIAMS, RS
通讯作者：
WILLIAMS, RS