Nanofibrous spongy microspheres for the delivery of hypoxia-primed human dental pulp stem cells to regenerate vascularized dental pulp.
Nanofibrous spongy microspheres for the delivery of hypoxia-primed human dental pulp stem cells to regenerate vascularized dental pulp.
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DOI：
10.1016/j.actbio.2016.01.032
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发表时间：
2016-03
影响因子：
9.7
通讯作者：
Ma PX
中科院分区：
文献类型：
--
作者：
Kuang R;Zhang Z;Jin X;Hu J;Shi S;Ni L;Ma PX
牙髓感染和坏死是广泛存在的疾病。传统的牙髓治疗会导致牙齿失活和变弱。在这项工作中，我们合成了一种新的星形聚合物，可以自组装成独特的纳米纤维海绵微球（NF-SMS），用于将人牙髓干细胞（hDPSCs）携带到牙髓腔中以再生活牙髓组织。结果发现，与对照细胞载体相比，NF-SMS显著增强了hDPSCs的附着、增殖、成牙分化和血管生成。此外，在3D缺氧培养中，NF-SMS促进了hdpsc血管内皮生长因子（VEGF）的表达。将缺氧诱导的hdpsc /NF-SMS复合物注射到清洁的兔磨牙牙髓腔中，用于小鼠皮下植入。4周后，与对照组（hDPSCs单独培养组、NF-SMS单独培养组和hDPSCs/NF-SMS预培养组）相比，缺氧组显著增强牙髓腔内血管生成，促进牙本质-牙髓界面上成牙釉质样细胞的形成。此外，在大鼠原位牙髓修复模型中，注射缺氧诱导的hdpsc /NF-SMS，使其充分填充牙髓腔，再生出血管丰富、组织结构与天然牙髓相似的牙髓样组织。
Dental pulp infection and necrosis are widespread diseases. Conventional endodontic treatments result in a devitalized and weakened tooth. In this work, we synthesized novel star-shaped polymer to self-assemble into unique nanofibrous spongy microspheres (NF-SMS), which were used to carry human dental pulp stem cells (hDPSCs) into the pulp cavity to regenerate living dental pulp tissues. It was found that NF-SMS significantly enhanced hDPSCs attachment, proliferation, odontogenic differentiation and angiogenesis, as compared to control cell carriers. Additionally, NF-SMS promoted vascular endothelial growth factor (VEGF) expression of hDPSCs in a 3D hypoxic culture. Hypoxia-primed hDPSCs/NF-SMS complexes were injected into the cleaned pulp cavities of rabbit molars for subcutaneous implantation in mice. After 4 weeks, the hypoxia group significantly enhanced angiogenesis inside the pulp chamber and promoted the formation of ondontoblast-like cells lining along the dentin-pulp interface, as compared to the control groups (hDPSCs alone group, NF-SMS alone group, and hDPSCs/NF-SMS group pre-cultured under normoxic conditions). Furthermore, in an in situ dental pulp repair model in rats, hypoxia-primed hDPSCs/NF-SMS were injected to fully fill the pulp cavity and regenerate pulp-like tissues with a rich vasculature and a histological structure similar to the native pulp.
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影响因子：
10
作者：
Kuang R;Zhang Z;Jin X;Hu J;Gupte MJ;Ni L;Ma PX
通讯作者：
Ma PX
影响因子：
20.3
作者：
Manalo, DJ;Rowan, A;Semenza, GL
通讯作者：
Semenza, GL
影响因子：
56.9
作者：
Matsumoto, K;Yoshitomi, H;Zaret, KS
通讯作者：
Zaret, KS
影响因子：
9.7
作者：
Blaker, JJ;Maquet, V;Nazhat, SN
通讯作者：
Nazhat, SN
影响因子：
4.2
作者：
Dammaschke, T;Steven, D;Reiner, KH
通讯作者：
Reiner, KH