Cultivation-independent methods applied to the microbial prospection of oil and gas in soil from a sedimentary basin in Brazil.
Cultivation-independent methods applied to the microbial prospection of oil and gas in soil from a sedimentary basin in Brazil.
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DOI：
10.1186/2191-0855-1-35
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发表时间：
2011-10-22
期刊：
影响因子：
3.7
通讯作者：
de Oliveira VM
中科院分区：
文献类型：
--
作者：
Miqueletto PB;Andreote FD;Dias AC;Ferreira JC;Dos Santos Neto EV;de Oliveira VM
油田构筑物上部可能发生气体泄漏，本应通过土壤细菌数量间接检测。这种微生物能够消耗这种天然气，支持石油和天然气微生物勘探(MPOG)方法。本工作的目的是通过克隆细菌和古生菌的16S rRNA基因和编码可溶性二铁单加氧酶(SDIMO)酶α亚基的分解代谢基因的文库，来表征石油(P)土壤样品中参与短链烷烃代谢的微生物群落，即甲烷、乙烷和丙烷。微生物群落表现出很高的细菌门放线菌门，占克隆总数的53%，以及来自古生物域的Cren考古群I.1b。对分解代谢基因的分析表明，与非含油(NP)土壤(Chao=2；Ace=0，Shannon=0.44)相比，P土壤中存在7个操作蛋白家族(OPF)，且丰富度(Chao=7；Ace=7.5)和多样性(Shannon=1.09)较高。与乙烯单加氧酶(EtnC)和甲烷单加氧酶(MmoX)编码基因相关的克隆只出现在P土壤中，短链碳氢化合物测定显示，P土壤中甲烷含量较高，乙烷和丙烷含量较低。实时定量聚合酶链式反应结果表明，SDIMO基因在所研究的土壤样本中的丰度很低。有必要对自然环境中的SDIMOS基因进行进一步研究，以揭示其仍然未知的多样性，并为预测退化人口提供可靠的工具。
The upper parts of oil field structures may leak gas which is supposed to be indirectly detected by the soil bacterial populations. Such microorganisms are capable of consuming this gas, supporting the Microbial Prospection of Oil and Gas (MPOG) methodology. The goal of the present work was to characterize microbial communities involved in short-chain alkane metabolism, namely methane, ethane and propane, in samples from a petroliferous (P) soil through clone libraries of the 16S rRNA gene of the Domains Bacteria and Archaea and the catabolic gene coding for the soluble di-iron monooxygenase (SDIMO) enzyme alpha subunit. The microbial community presented high abundance of the bacterial phylum Actinobacteria, which represented 53% of total clones, and the Crenarchaeota group I.1b from the Archaea Domain. The analysis of the catabolic genes revealed the occurrence of seven Operational Protein Families (OPF) and higher richness (Chao = 7; Ace = 7.5) and diversity (Shannon = 1.09) in P soil when compared with a non-petroliferous (Np) soil (Chao = 2; Ace = 0, Shannon = 0.44). Clones related to the ethene monooxygenase (EtnC) and methane monooxygenase (MmoX) coding genes occurred only in P soil, which also presented higher levels of methane and lower levels of ethane and propane, revealed by short-chain hydrocarbon measures. Real-time PCR results suggested that the SDIMO genes occur in very low abundance in the soil samples under study. Further investigations on SDIMOs genes in natural environments are necessary to unravel their still uncharted diversity and to provide reliable tools for the prospection of degrading populations.
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影响因子：
5.1
作者：
Casamayor, EO;Massana, R;Pedrós-Alió, C
通讯作者：
Pedrós-Alió, C
DOI：
10.1007/s10482-008-9227-1
发表时间：
2008-06-01
影响因子：
2.6
作者：
Holmes, Andrew J.;Coleman, Nicholas V.
通讯作者：
Coleman, Nicholas V.
影响因子：
5.8
作者：
Chakraborty, Sinjini;Mukherji, Soumyo;Mukherji, Suparna
通讯作者：
Mukherji, Suparna
影响因子：
2.8
作者：
Cooley, Richard B.;Dubbel, Bradley L.;Arp, Daniel J.
通讯作者：
Arp, Daniel J.
影响因子：
4.8
作者：
Fierer, Noah;Bradford, Mark A.;Jackson, Robert B.
通讯作者：
Jackson, Robert B.