Cell tracing dyes significantly change single cell mechanics.
Cell tracing dyes significantly change single cell mechanics.
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DOI：
10.1021/jp8103358
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发表时间：
2009-05-07
期刊：
影响因子：
--
通讯作者：
Liu GY
中科院分区：
文献类型：
--
作者：
Lulevich V;Shih YP;Lo SH;Liu GY
细胞示踪染料在细胞生物学研究中非常频繁地使用，因为它们提供不损害细胞功能（例如生长和增殖）的高灵敏度荧光标记。在许多关于细胞粘附和力学的研究中，荧光染料已经被采用，并假设对结果的影响很小。使用我们团队开发的单细胞压缩技术，研究了MDA-MB-468和MLC-SV 40细胞的单细胞力学随染料摄取的变化。细胞示踪染料使活细胞硬度增加3-6倍，使细胞与探针的粘附增加7倍。这些结果表明，比毒素，如凝血酶更显着的效果。一个简单的分析模型推导出，使提取的杨氏模量的细胞膜和细胞骨架的力-变形的个人细胞测量的配置文件。膜的杨氏模量增加3-7倍，这比细胞骨架的杨氏模量（1.1-3.4倍）更显著。我们提出，细胞力学的变化后，加入荧光示踪染料主要是由于两亲性染料分子纳入细胞质膜，这增加了磷脂链之间的横向相互作用，从而提高了它们的刚性和粘附性。
Cell tracing dyes are very frequently utilized in cellular biology research because they provide highly sensitive fluorescent tags that do not compromise cellular functions such as growth and proliferation. In many investigations concerning cellular adhesion and mechanics, fluorescent dyes have been employed with the assumption of little impact on the results. Using the single-cell compression technique developed by our team, the single-cell mechanics of MDA-MB-468 and MLC-SV40 cells were investigated as a function of dye uptake. Cell tracing dyes increase living cell stiffness 3-6 times and cell-to-probe adhesion up to 7 times. These results suggest a more significant effect than toxins, such as Thrombin. A simple analytical model was derived to enable the extraction of the Young’s moduli of the cell membrane and cytoskeleton from the force-deformation profiles measured for individual cells. The increase in Young’s modulus of the membrane is 3-7 times, which is more significant than that of the cytoskeleton (1.1-3.4 times). We propose that changes in cell mechanics upon the addition of fluorescent tracing dye are primarily due to incorporation of amphiphilic dye molecules into the cellular plasma membrane, which increases the lateral interaction among phospholipid chains and thus enhances their rigidity and adhesion.
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影响因子：
3.9
作者：
Lulevich, VV;Vinogradova, OI
通讯作者：
Vinogradova, OI
影响因子：
3.4
作者：
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通讯作者：
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影响因子：
--
作者：
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通讯作者：
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影响因子：
56.9
作者：
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通讯作者：
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影响因子：
2.2
作者：
DECLERCK, LS;BRIDTS, CH;STEVENS, WJ
通讯作者：
STEVENS, WJ