Regulated proenkephalin expression in human skin and cultured skin cells.
Regulated proenkephalin expression in human skin and cultured skin cells.
复制标题
DOI：
10.1038/jid.2010.376
复制
发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
皮肤通过局部神经免疫内分泌系统的协调作用对环境压力源做出反应。尽管其中一些反应涉及阿片受体，但人们对皮肤脑啡肽原表达、其环境调节和病理学改变知之甚少。本研究的目的是评估正常和病理皮肤以及分离的黑色素细胞、角质形成细胞、成纤维细胞和黑色素瘤细胞中脑啡肽原的调节表达。脑啡肽原基因和蛋白在皮肤和培养细胞中表达，其中在成纤维细胞和角质形成细胞中表达显着。质谱证实皮肤中存在亮氨酸脑啡肽和甲硫氨酸脑啡肽。 UVR、Toll 样受体 (TLR)4 和 TLR2 激动剂以时间和剂量依赖性方式刺激黑素细胞和角质形成细胞中脑啡肽原基因的表达。原位 Met/Leu-脑啡肽在毛囊外根鞘的表皮分化角质形成细胞、小汗腺的肌上皮细胞以及分隔上皮和间质成分的基底膜/基底层中表达。 Met/Leu-脑啡肽的表达在病理皮肤中发生改变，在牛皮癣中增加，在黑素细胞肿瘤中减少。人类皮肤不仅表达脑啡肽原，而且这种表达会因压力刺激而上调，并可能因病理状况而改变。
Skin responds to environmental stressors via coordinated actions of the local neuroimmunoendocrine system. Although some of these responses involve opioid receptors, little is known about cutaneous proenkephalin expression, its environmental regulation, and alterations in pathology. The objective of this study was to assess regulated expression of proenkephalin in normal and pathological skin and in isolated melanocytes, keratinocytes, fibroblasts, and melanoma cells. The proenkephalin gene and protein were expressed in skin and cultured cells, with significant expression in fibroblasts and keratinocytes. Mass spectroscopy confirmed Leu- and Met-enkephalin in skin. UVR, Toll-like receptor (TLR)4, and TLR2 agonists stimulated proenkephalin gene expression in melanocytes and keratinocytes in a time- and dose-dependent manner. In situ Met/Leu-enkephalin peptides were expressed in differentiating keratinocytes of the epidermis in the outer root sheath of the hair follicle, in myoepithelial cells of the eccrine gland, and in the basement membrane/basal lamina separating epithelial and mesenchymal components. Met/Leu-enkephalin expression was altered in pathological skin, increasing in psoriasis and decreasing in melanocytic tumors. Not only does human skin express proenkephalin, but this expression is upregulated by stressful stimuli and can be altered by pathological conditions.
登录
查看更多内容
影响因子：
6.5
作者：
Bigliardi, PL;Mei, BQ;Rufli, T
通讯作者：
Rufli, T
影响因子：
6.5
作者：
Bigliardi, PL;Sumanovski, LT;Bigliardi-Qi, M
通讯作者：
Bigliardi-Qi, M
影响因子：
13.8
作者：
Feldman, SR;Liguori, A;Kaur, M
通讯作者：
Kaur, M
影响因子：
4.8
作者：
Kieffer, AE;Goumon, Y;Metz-Boutigue, MH
通讯作者：
Metz-Boutigue, MH
影响因子：
6.5
作者：
Kauser, S;Schallreuter, KU;Tobin, DJ
通讯作者：
Tobin, DJ