Karyotype Evolution in 10 Pinniped Species: Variability of Heterochromatin versus High Conservatism of Euchromatin as Revealed by Comparative Molecular Cytogenetics.
Karyotype Evolution in 10 Pinniped Species: Variability of Heterochromatin versus High Conservatism of Euchromatin as Revealed by Comparative Molecular Cytogenetics.
复制标题
10种鳍足动物的核型进化：比较分子细胞遗传学揭示的异染色质变异性与常染色质高度保守性。
DOI：
10.3390/genes11121485
复制
发表时间：
2020-12-10
期刊：
影响因子：
3.5
通讯作者：
Graphodatsky AS
中科院分区：
文献类型：
--
作者：
Beklemisheva VR;Perelman PL;Lemskaya NA;Proskuryakova AA;Serdyukova NA;Burkanov VN;Gorshunov MB;Ryder O;Thompson M;Lento G;O'Brien SJ;Graphodatsky AS
本文利用人类、家犬和石貂的全染色体绘画探针研究了鳍足类的核型演化，获得了Odobenidae（Odobenus rosmarus）、Phocalae（Phoca vitulina、Phoca largha、Phoca hispida、Pusa sibirica、Erignathus vitatus）和Otariidae（Eumetopias jubatus、Callorhinus ursinus、Phocartos hookeri和Arctocephalus forsteri）物种之间的比较染色体图谱。用端粒重复序列和核糖体DNA探针以及CBG（通过氢氧化钡处理后Giemsa染色显示C带）和CDAG（G显带后的色霉素A3-DAPI）方法评估染色体的结构和功能特征。我们证明了多样性的异染色质之间的鳍足类核型的定位，大小和核苷酸组成。首次揭示了一种在Otariidae和Odobenidae中常见的染色体内重排。我们假设，在分析的鳍足类的进化保守的片段的顺序是相同的顺序提出的祖先食肉目核型（2n = 38）。鳍足类保守基因组的进化伴随着一些融合事件（每1000万年不到一次重排）和新的染色体内变化，包括新的着丝粒和着丝粒周围倒位/着丝粒重新定位的出现。由组成性异染色质变异驱动的鳍足类核型的种间多样性可能在鳍足类的核型进化中发挥了重要作用，从而导致了鳍足类染色体组的差异。
Pinnipedia karyotype evolution was studied here using human, domestic dog, and stone marten whole-chromosome painting probes to obtain comparative chromosome maps among species of Odobenidae (Odobenus rosmarus), Phocidae (Phoca vitulina, Phoca largha, Phoca hispida, Pusa sibirica, Erignathus barbatus), and Otariidae (Eumetopias jubatus, Callorhinus ursinus, Phocarctos hookeri, and Arctocephalus forsteri). Structural and functional chromosomal features were assessed with telomere repeat and ribosomal-DNA probes and by CBG (C-bands revealed by barium hydroxide treatment followed by Giemsa staining) and CDAG (Chromomycin A3-DAPI after G-banding) methods. We demonstrated diversity of heterochromatin among pinniped karyotypes in terms of localization, size, and nucleotide composition. For the first time, an intrachromosomal rearrangement common for Otariidae and Odobenidae was revealed. We postulate that the order of evolutionarily conserved segments in the analyzed pinnipeds is the same as the order proposed for the ancestral Carnivora karyotype (2n = 38). The evolution of conserved genomes of pinnipeds has been accompanied by few fusion events (less than one rearrangement per 10 million years) and by novel intrachromosomal changes including the emergence of new centromeres and pericentric inversion/centromere repositioning. The observed interspecific diversity of pinniped karyotypes driven by constitutive heterochromatin variation likely has played an important role in karyotype evolution of pinnipeds, thereby contributing to the differences of pinnipeds’ chromosome sets.
登录
查看更多内容
DOI：
10.1159/000056788
发表时间：
2000-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
Graphodatsky, AS;Yang, F;Ferguson-Smith, MA
通讯作者：
Ferguson-Smith, MA
影响因子：
1.7
作者：
Graphodatsky, AS;Yang, F;Ferguson-Smith, MA
通讯作者：
Ferguson-Smith, MA
影响因子：
7
作者：
Capozzi, Oronzo;Purgato, Stefania;Rocchi, Mariano
通讯作者：
Rocchi, Mariano
影响因子：
1.6
作者：
ARNASON, U;WIDEGREN, B
通讯作者：
WIDEGREN, B
DOI：
10.1139/z67-088
发表时间：
1967-01-01
期刊：
CANADIAN JOURNAL OF ZOOLOGY - BACK YEAR PROJECT
影响因子：
--
作者：
FAY, FH;RAUSCH, VR;FELTZ, ET
通讯作者：
FELTZ, ET