Molecular differences between the rostral and caudal halves of the sclerotome in the chick embryo.
Molecular differences between the rostral and caudal halves of the sclerotome in the chick embryo.
复制标题
鸡胚胎中骨节的头端和尾端之间的分子差异。
作者：
W. Norris;Claudio D. Stern;R. Keynes
众所周知，大多数脊椎动物胚胎中的神经脊细胞迁移和运动轴突长出都是分段的，这是因为相邻的硬化体对它们的分布施加了限制，每个硬化体都分为吻侧和尾侧两个部分。尾侧的一半不允许脊移动或轴突长出，而吻侧的一半则允许。本文用双向SDS-聚丙烯酰胺凝胶电泳法研究了20~32对体节期间鸡胚菌核两半的蛋白质和糖蛋白的表达。我们发现，表达模式是复杂的，多肽和糖蛋白在空间和时间上都不同：在菌核体差异表达的多肽和糖蛋白中，有些是定量的，另一些是定性的。有些大分子会随着发育年龄的增长而改变其空间分布，有些则会随着胚胎年龄的增长而出现或消失。
It is known that both neural crest cell migration and motor axon outgrowth in most vertebrate embryos are segmented because of restrictions imposed upon their distribution by the neighbouring sclerotomes, each of which is divided into a rostral and a caudal half. The caudal half does not allow crest migration or axon outgrowth, while the rostral half does. In this paper, we investigate the expression of proteins and glycoproteins in the two halves of the sclerotome of the chick embryo at stages between 20 and 32 pairs of somites by two-dimensional SDS-polyacrylamide gel electrophoresis. We find that the patterns of expression are complex, and that polypeptides and glycoproteins vary both spatially and temporally: of those that are expressed differentially by the sclerotome, some differ quantitatively and others qualitatively. Some macromolecules change their spatial distribution with developmental age, and some appear or disappear as the embryos become older.
轴突生长和突触发生过程中 NCAM 在雏鸡后肢的分布。
影响因子：
2.7
作者：
Tosney,KW;Watanabe,M;Landmesser,L;Rutishauser,U
通讯作者：
Rutishauser,U