Diffusion tensor imaging detects axonal injury in a mouse model of repetitive closed-skull traumatic brain injury.
Diffusion tensor imaging detects axonal injury in a mouse model of repetitive closed-skull traumatic brain injury.
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DOI：
10.1016/j.neulet.2012.02.024
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发表时间：
2012-04-04
影响因子：
2.5
通讯作者：
Brody DL
中科院分区：
文献类型：
--
作者：
Bennett RE;Mac Donald CL;Brody DL
轻度创伤性脑损伤（TBI）在运动员、军人和老年人中很常见，越来越多的证据表明这些损伤对健康有长期影响。然而，在体内检测这些轻度损伤的困难是理解潜在病理和治疗轻度TBI的重大障碍。在接下来的实验中，我们报告了小鼠轻度重复性闭合性颅脑损伤模型的弥散张量成像（DTI）和组织学分析结果。组织学标记包括银染色和淀粉样前体蛋白（APP）免疫组化检测轴突损伤，Iba-1免疫组化评估小胶质细胞活化。损伤后24小时，在组织学上未见银染色或小胶质细胞异常前，白质内DTI参数未见明显变化。损伤后7天，我们观察到轴向扩散率和平均扩散率降低。第7天的相对各向异性与银染色程度密切相关。有趣的是，在检查的任何时间点都没有观察到APP。除了白质改变外，同侧皮质的平均弥漫性在24小时内升高，但在7天后恢复到假手术水平。总之，这表明尽管缺乏传统的APP病理，DTI是一种检测轴突损伤的敏感方法。此外，这反映了需要更好地了解轻度TBI中DTI信号变化的组织学基础。
Mild traumatic brain injuries (TBI) are common in athletes, military personnel, and the elderly, and increasing evidence indicates that these injuries have long-term health effects. However, the difficulty in detecting these mild injuries in vivo is a significant impediment to understanding the underlying pathology and treating mild TBI. In the following experiments, we present the results of diffusion tensor imaging (DTI) and histological analysis of a model of mild repetitive closed-skull brain injury in mouse. Histological markers used included silver staining and amyloid precursor protein (APP) immunohistochemistry to detect axonal injury, and Iba-1 immunohistochemistry to assess microglial activation. At 24 hours post-injury, before silver staining or microglial abnormalities were apparent by histology, no significant changes in any of the DTI parameters were observed within white matter. At 7 days post-injury we observed a reduction in axial and mean diffusivity. Relative anisotropy at 7 days correlated strongly with the degree of silver staining. Interestingly, APP was not observed at any timepoint examined. In addition to the white matter alterations, mean diffusivity was elevated in ipsilateral cortex at 24 hours but returned to sham levels by 7 days. Altogether, this demonstrates that DTI is a sensitive method for detecting axonal injury despite a lack of conventional APP pathology. Further, this reflects a need to better understand the histological basis for DTI signal changes in mild TBI.
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影响因子：
5.3
作者：
Mac Donald, C. L.;Dikranian, K.;Brody, D. L.
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.2
作者：
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通讯作者：
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