Radial diffusivity predicts demyelination in ex vivo multiple sclerosis spinal cords.
Radial diffusivity predicts demyelination in ex vivo multiple sclerosis spinal cords.
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DOI：
10.1016/j.neuroimage.2011.01.007
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发表时间：
2011-04-15
期刊：
影响因子：
5.7
通讯作者：
Benzinger, Tammie L.
中科院分区：
文献类型：
--
作者：
Klawiter, Eric C.;Schmidt, Robert E.;Trinkaus, Kathryn;Liang, Hsiao-Fang;Budde, Matthew D.;Naismith, Robert T.;Song, Sheng-Kwei;Cross, Anne H.;Benzinger, Tammie L.
扩散张量成像（DTI）与组织化学染色在离体多发性硬化（MS）人颈脊髓脱髓鞘和轴突损伤中的相关性。在MS中，脱髓鞘、轴突变性和炎症在不同程度上促成疾病发病机制。基于急性损伤和组织病理学相关性的体内动物研究，我们假设DTI可以区分人类的轴突和髓鞘病变。在4.7T下对9例MS和5例正常对照的固定颈脊髓块进行DTI。然后将切片用Luxol坚牢蓝（LFB）、Bielschowsky银和苏木精和伊红（H&E）染色。将感兴趣区域（ROI）半定量地分级为正常髓鞘形成、轻度（<50%）脱髓鞘或中度-重度（>50%）脱髓鞘。在Bielschowsky银上手动测定相应的轴突计数。将ROI映射到共配准的DTI参数切片。评价的DTI参数包括表观扩散系数（ADC）、相对各向异性（RA）、轴向扩散率和径向扩散率的标准定量评估。使用线性混合模型在组织化学分级和DTI参数之间进行统计相关性。结果：在MS受试者的ROI内，径向扩散率的增加与脱髓鞘严重程度的恶化有关。与正常区域和轻度脱髓鞘区域相比，中重度脱髓鞘区域的相对各向异性降低。径向扩散率，ADC，和RA变得越来越改变恶化轴突计数的四分位数。轴向扩散率与轴突密度无关（p=0.091）。增加的径向扩散率可以作为脱髓鞘的替代物。然而，径向扩散率也随着轴突损伤而改变，这表明该测量在慢性人类MS组织中没有病理特异性。我们建议径向扩散率可以作为慢性MS病变内整体组织完整性的标志。本研究为正在进行的MS的活体DTI研究提供了病理学基础。
Correlation of diffusion tensor imaging (DTI) with histochemical staining for demyelination and axonal damage in multiple sclerosis (MS) ex vivo human cervical spinal cords. In MS, demyelination, axonal degeneration, and inflammation contribute to disease pathogenesis to variable degrees. Based upon in vivo animal studies with acute injury and histopathologic correlation, we hypothesized that DTI can differentiate between axonal and myelin pathologies within humans. DTI was performed at 4.7 Tesla on 9 MS and 5 normal control fixed cervical spinal cord blocks following autopsy. Sections were then stained for Luxol fast blue (LFB), Bielschowsky silver, and hematoxylin and eosin (H&E). Regions of interest (ROIs) were graded semi-quantitatively as normal myelination, mild (<50%) demyelination, or moderate-severe (>50%) demyelination. Corresponding axonal counts were manually determined on Bielschowsky silver. ROIs were mapped to co-registered DTI parameter slices. DTI parameters evaluated included standard quantitative assessments of apparent diffusion coefficient (ADC), relative anisotropy (RA), axial diffusivity and radial diffusivity. Statistical correlations were made between histochemical gradings and DTI parameters using linear mixed models. Results: Within ROIs in MS subjects, increased radial diffusivity distinguished worsening severities of demyelination. Relative anisotropy was decreased in the setting of moderate-severe demyelination compared to normal areas and areas of mild demyelination. Radial diffusivity, ADC, and RA became increasingly altered within quartiles of worsening axonal counts. Axial diffusivity did not correlate with axonal density (p=0.091). Increased radial diffusivity can serve as a surrogate for demyelination. However, radial diffusivity was also altered with axon injury, suggesting that this measure is not pathologically specific within chronic human MS tissue. We propose that radial diffusivity can serve as a marker of overall tissue integrity within chronic MS lesions. This study provides pathologic foundation for on-going in vivo DTI studies in MS.
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影响因子：
5.7
作者：
Schmierer, Klaus;Wheeler-Kingshott, Claudia A. M.;Boulby, Phil A.;Scaravilli, Francesco;Altmann, Daniel R.;Barker, Gareth J.;Tofts, Paul S.;Miller, David H.
通讯作者：
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影响因子：
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作者：
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14.5
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影响因子：
14.5
作者：
BARNES, D;MUNRO, PMG;MCDONALD, WI
通讯作者：
MCDONALD, WI
影响因子：
9.9
作者：
Naismith, R. T.;Xu, J.;Cross, A. H.
通讯作者：
Cross, A. H.