Intestinal Dysbiosis in Young Cystic Fibrosis Rabbits.
Intestinal Dysbiosis in Young Cystic Fibrosis Rabbits.
复制标题
囊性纤维化幼兔肠道生态失调。
DOI：
10.3390/jpm11020132
复制
发表时间：
2021-02-16
影响因子：
--
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Liang X;Bouhamdan M;Hou X;Zhang K;Song J;Hao K;Jin JP;Zhang Z;Xu J
囊性纤维化（CF）患者常经历胃肠道（GI）异常。近年来，肠道微生物群被认为是cf相关胃肠道并发症发生的一个因素，因此代表了一个潜在的治疗靶点。我们最近建立了CF兔模型，该模型显示出许多与人类患者相似的病理变化，包括肠梗阻。在这里，我们研究了没有抗生素治疗的幼年CF兔的粪便微生物组。收集7 ~ 9周龄CF兔（n = 7）和年龄匹配的野生型（WT）兔（n = 6）的粪便样本。通过16S rRNA基因的iTag测序研究微生物组，并使用PICRUSt预测功能谱。与儿童CF患者的报道一致，CF家兔的粪便微生物组的丰富度和多样性低于WT家兔，具有明显的分类学和推断功能失调。我们的工作确定了一种新的CF动物模型，表现为肠道生态失调表型。该模型系统可促进cf相关胃肠道疾病新疗法的研究和开发。
Individuals with cystic fibrosis (CF) often experience gastrointestinal (GI) abnormalities. In recent years, the intestinal microbiome has been postulated as a contributor to the development of CF-associated GI complications, hence representing a potential therapeutic target for treatment. We recently developed a rabbit model of CF, which is shown to manifest many human patient-like pathological changes, including intestinal obstruction. Here, we investigated the feces microbiome in young CF rabbits in the absence of antibiotics treatment. Stool samples were collected from seven- to nine-week-old CF rabbits (n = 7) and age-matched wild-type (WT) rabbits (n = 6). Microbiomes were investigated by iTag sequencing of 16S rRNA genes, and functional profiles were predicted using PICRUSt. Consistent with reports of those in pediatric CF patients, the fecal microbiomes of CF rabbits are of lower richness and diversity than that of WT rabbits, with a marked taxonomic and inferred functional dysbiosis. Our work identified a new CF animal model with the manifestation of intestinal dysbiosis phenotype. This model system may facilitate the research and development of novel treatments for CF-associated gastrointestinal diseases.
登录
查看更多内容
影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.2
作者：
Antosca, Katherine M.;Chernikova, Diana A.;Madan, Juliette C.
通讯作者：
Madan, Juliette C.
影响因子：
5.5
作者：
Laselva, Onofrio;Stone, Tracy A.;Deber, Charles M.
通讯作者：
Deber, Charles M.