Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family.
Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family.
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人嗜酸性粒细胞衍生的神经毒素的分子克隆：核糖核酸酶基因家族的成员。
DOI：
10.1073/pnas.86.12.4460
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发表时间：
1989
影响因子：
11.1
通讯作者：
Ackerman,SJ
中科院分区：
文献类型：
--
作者：
Rosenberg,HF;Tenen,DG;Ackerman,SJ
我们分离出了人嗜酸性粒细胞衍生神经毒素 (EDN) 的 725 个碱基对 cDNA 克隆。 EDN 是嗜酸性粒细胞大特异性颗粒的一种独特阳离子蛋白，主要因其在实验动物中诱导共济失调、麻痹和中枢神经系统细胞变性（戈登现象）而闻名。开放阅读框编码134个氨基酸的成熟多肽，分子量为15.5kDa，具有27个残基的氨基末端疏水前导序列。成熟多肽的序列与报道的人尿核糖核酸酶的序列相同[Beintema，J.J.，Hofsteenge，J.，Iwama，M.，Morita，T.，Ohgi，K.，Irie，M.，Sugiyama，R.H.，Schieven，G.L.，Dekker，C.A.&Glitz，D.G.(1988)Biochemistry 27， 4530-4538]和人肝核糖核酸酶的氨基末端序列[Sorrentino, S., Tucker, G. K. & Glitz, D. G. (1988) J. Biol. 4530-4538]化学。 263、16125-16131]；该cDNA在第7位编码色氨酸，该色氨酸以前在EDN或尿和肝核糖核酸酶的氨基酸序列中未被识别。 EDN和相关的颗粒蛋白、嗜酸性粒细胞阳离子蛋白均具有核糖核酸水解活性； EDN、嗜酸性粒细胞阳离子蛋白、来自肝脏、尿液和胰腺的核糖核酸酶以及血管生成素之间的序列相似性定义了核糖核酸酶多基因家族。在未诱导的 HL-60 细胞中检测到编码 EDN 的 mRNA，并且在用 B 细胞生长因子 2/白细胞介素 5 诱导的嗜酸性粒细胞分化和用二甲亚砜诱导的中性粒细胞分化的细胞中上调。尽管在中性粒细胞提取物中未发现 EDN 样神经毒性活性，但在成熟中性粒细胞中检测到了 EDN mRNA。这些结果表明中性粒细胞含有与 EDN 密切相关或相同的蛋白质。
We have isolated a 725-base-pair cDNA clone for human eosinophil-derived neurotoxin (EDN). EDN is a distinct cationic protein of the eosinophil's large specific granule known primarily for its ability to induce ataxia, paralysis, and central nervous system cellular degeneration in experimental animals (Gordon phenomenon). The open reading frame encodes a 134-amino acid mature polypeptide with a molecular mass of 15.5 kDa and a 27-residue amino-terminal hydrophobic leader sequence. The sequence of the mature polypeptide is identical to that reported for human urinary ribonuclease [Beintema, J. J., Hofsteenge, J., Iwama, M., Morita, T., Ohgi, K., Irie, M., Sugiyama, R. H., Schieven, G. L., Dekker, C. A. & Glitz, D. G. (1988) Biochemistry 27, 4530-4538] and to the amino-terminal sequence of human liver ribonuclease [Sorrentino, S., Tucker, G. K. & Glitz, D. G. (1988) J. Biol. Chem. 263, 16125-16131]; the cDNA encodes a tryptophan in position 7, which was previously unidentified in the amino acid sequences of EDN or the urinary and liver ribonucleases. Both EDN and the related granule protein, eosinophil cationic protein, have ribonucleolytic activity; sequence similarities among EDN, eosinophil cationic protein, ribonucleases from liver, urine, and pancreas, and angiogenin define a ribonuclease multigene family. mRNA encoding EDN was detected in uninduced HL-60 cells and was up-regulated in cells induced toward eosinophilic differentiation with B-cell growth factor 2/interleukin 5 and toward neutrophilic differentiation with dimethyl sulfoxide. EDN mRNA was detected in mature neutrophils even though EDN-like neurotoxic activity is not found in neutrophil extracts. These results suggest that neutrophils contain a protein that is closely related or identical to EDN.
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DOI：
10.1016/s0021-9258(18)37567-7
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Salvatore SorrentinoS;Gabrielle Kapor Tucker;Dohn;Glitz
通讯作者：
Glitz
哺乳动物溶核酶。
DOI：
10.1016/s0079-6603(08)60470-5
发表时间：
1977
期刊：
Progress in nucleic acid research and molecular biology
影响因子：
--
作者：
H. Sierakowska;D. Shugar
通讯作者：
D. Shugar
DOI：
10.1016/s0006-291x(86)80310-2
发表时间：
1986-09-30
影响因子：
3.1
作者：
GULLBERG, U;WIDEGREN, B;OLSSON, I
通讯作者：
OLSSON, I
影响因子：
4.3
作者：
H. Sambrook
通讯作者：
H. Sambrook
影响因子：
2.9
作者：
STRYDOM, DJ;FETT, JW;VALLEE, BL
通讯作者：
VALLEE, BL