Analyses of murine GBP homology clusters based on in silico, in vitro and in vivo studies.
Analyses of murine GBP homology clusters based on in silico, in vitro and in vivo studies.
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基于硅，体外和体内研究的鼠同源物簇的分析。
DOI：
10.1186/1471-2164-9-158
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发表时间：
2008-04-10
期刊：
影响因子：
4.4
通讯作者：
Beer, Sandra
中科院分区：
文献类型：
--
作者：
Kresse, Alexandra;Konermann, Carolin;Degrandi, Daniel;Beuter-Gunia, Cornelia;Wuerthner, Jan;Pfeffer, Klaus;Beer, Sandra
病原体和宿主之间的相互作用导致抗微生物宿主效应分子的大量上调。其中，65 kDa的鸟苷酸结合蛋白（GBP）作为宿主效应分子库的复杂组分是令人感兴趣的候选者。GBP家族成员在脊椎动物中高度保守。先前的报道表明人GBP 1（hGBP 1）和鼠GBP 2（mGBP 2）具有抗病毒活性。我们最近发现，不同的小鼠GBP（mGBP）家族成员在弓形虫感染后高度上调，并定位于细胞内原生动物T。刚地。此外，我们在小鼠65 kDa GBP家族中鉴定了5个新的mGBP家族成员。在这里，我们确定了一个新的mGBP位点命名为mGbp 11。基于细菌人工染色体（BAC）、表达序列标签（EST）和RT-PCR分析，本研究提供了对mGBP的基因组定位和组织的详细了解。这些分析揭示了3号染色体上的一个166 kb的跨越区域，包含5个转录的mGBP（mGbp 1、mGbp 2、mGbp 3、mGbp 5和mGbp 7）和一个假基因（pseudomGbp 1），以及5号染色体上的一个332 kb的跨越区域，由6个转录的mGBP（mGbp 4、mGbp 6、mGbp 8、mGbp 9、mGbp 10和mGbp 11）和一个假基因（pseudomgbp 2）组成。除了在编码序列内具有65%至98%的惊人高同源性之外，5号染色体簇上的mGBP还表现出高度同源的外显子-内含子结构，而3号染色体上的mGBP揭示了更发散的外显子-内含子结构。这项研究详细介绍了mGBP的全面基因组组织，并表明不断变化的微生物环境对该基因家族施加了进化压力，导致多个基因扩增。本文的链接列表可以在可用性和要求部分找到。
The interactions between pathogens and hosts lead to a massive upregulation of antimicrobial host effector molecules. Among these, the 65 kDa guanylate binding proteins (GBPs) are interesting candidates as intricate components of the host effector molecule repertoire. Members of the GBP family are highly conserved in vertebrates. Previous reports indicate an antiviral activity of human GBP1 (hGBP1) and murine GBP2 (mGBP2). We recently demonstrated that distinct murine GBP (mGBP) family members are highly upregulated upon Toxoplasma gondii infection and localize around the intracellular protozoa T. gondii. Moreover, we characterised five new mGBP family members within the murine 65 kDa GBP family. Here, we identified a new mGBP locus named mGbp11. Based on bacterial artificial chromosome (BAC), expressed sequence tag (EST), and RT-PCR analyses this study provides a detailed insight into the genomic localization and organization of the mGBPs. These analyses revealed a 166-kb spanning region on chromosome 3 harboring five transcribed mGBPs (mGbp1, mGbp2, mGbp3, mGbp5, and mGbp7) and one pseudogene (pseudomGbp1), as well as a 332-kb spanning region on chromosome 5 consisting of six transcribed mGBPs (mGbp4, mGbp6, mGbp8, mGbp9, mGbp10, and mGbp11), and one pseudogene (pseudomgbp2). Besides the strikingly high homology of 65% to 98% within the coding sequences, the mGBPs on chromosome 5 cluster also exhibit a highly homologous exon-intron structure whereas the mGBP on chromosome 3 reveals a more divergent exon-intron structure. This study details the comprehensive genomic organization of mGBPs and suggests that a continuously changing microbial environment has exerted evolutionary pressure on this gene family leading to multiple gene amplifications. A list of links for this article can be found in the Availability and requirements section.
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影响因子：
2.3
作者：
Nguyen, TT;Hu, Y;Smith, JB
通讯作者：
Smith, JB
影响因子：
6
作者：
Lubeseder-Martellato, C;Guenzi, E;Stürzl, M
通讯作者：
Stürzl, M
影响因子：
5.3
作者：
DECKER, T;LEW, DJ;DARNELL, JE
通讯作者：
DARNELL, JE
影响因子：
4.8
作者：
Gorbacheva, VY;Lindner, D;Vestal, DJ
通讯作者：
Vestal, DJ
影响因子：
5.3
作者：
LEW, DJ;DECKER, T;DARNELL, JE
通讯作者：
DARNELL, JE