Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6).
Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6).
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DOI：
10.1038/s41598-018-34860-3
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发表时间：
2018-11-08
影响因子：
4.6
通讯作者：
Tanaka T
中科院分区：
文献类型：
--
作者：
Hernandez EP;Kusakisako K;Talactac MR;Galay RL;Yoshii K;Tanaka T
铁是细胞非常重要的营养物质；然而，它也可能导致致命的影响，因为它能够引发氧化应激。由于蜱虫从血液中摄入大量铁，因此它们利用多种机制来应对氧化应激。一种机制是铁蛋白及其控制蛋白 (IRP) 的铁螯合。由于 IRP 活性取决于亚铁浓度，因此我们尝试通过在不同时间点将肩突硬蜱胚胎来源的细胞系 (ISE6) 暴露于不同浓度的硫酸亚铁来诱导细胞内铁蛋白 (FER1) 蛋白表达。正如在蛋白质印迹和间接免疫荧光抗体测试中观察到的那样，我们能够在暴露于 2mM 硫酸亚铁 48 小时后诱导 FER1 蛋白。这可能表明产生的 FER1 可能是 FER1 mRNA 释放 IRP 导致其翻译的产物。 FER1 通过转染 dsRNA 进行 RNA 干扰，导致 ISE6 细胞死亡率增加和细胞增殖减少。总体而言，ISE6 细胞可能是进一步了解 FER1 作用机制的良好工具，不仅在硬蜱中，而且在其他蜱种中也是如此。
Iron is a very important nutrient for cells; however, it could also cause fatal effects because of its capability to trigger oxidative stress. Due to high exposure to iron from their blood diet, ticks make use of several mechanisms to cope up with oxidative stress. One mechanism is iron sequestration by ferritin and its control protein (IRP). Since the IRP activity is dependent on the ferrous iron concentration, we tried to induce intracellular ferritin (FER1) protein expression by exposing Ixodes scapularis embryo-derived cell line (ISE6) to different concentrations of ferrous sulphate at different time points. We were able to induce FER1 protein after exposure to 2 mM of ferrous sulphate for 48 h, as observed in both Western blotting and indirect immunofluorescent antibody tests. This could indicate that the FER1 produced could be a product of the release of IRPs from the FER1 mRNA leading to its translation. The RNA interference of FER1, through the transfection of dsRNA, led to an increase in mortality and decrease in the cellular proliferation of ISE6 cells. Overall, ISE6 cells could be a good tool in further understanding the mechanism of FER1 action, not just in Ixodes ticks but in other tick species as well.
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DOI：
10.1073/pnas.0305888101
发表时间：
2004-04-27
影响因子：
11.1
作者：
Thorpe, GW;Fong, CS;Dawes, IW
通讯作者：
Dawes, IW
DOI：
10.1073/pnas.0601471103
发表时间：
2006-04-11
影响因子：
11.1
作者：
Missirlis, F;Holmberg, S;Law, JH
通讯作者：
Law, JH
DOI：
10.1016/j.bbamcr.2004.01.004
发表时间：
2004-05-03
影响因子：
5.1
作者：
Santamaria, R;Irace, C;Colonna, A
通讯作者：
Colonna, A
细胞死亡的基本与附件方面：NCCD 2015的建议。
影响因子：
12.4
作者：
通讯作者：
--
影响因子：
6.4
作者：
Hunter RC;Asfour F;Dingemans J;Osuna BL;Samad T;Malfroot A;Cornelis P;Newman DK
通讯作者：
Newman DK