Low let-7d microRNA levels in chick embryos enhance innate immunity against Mycoplasma gallisepticum by suppressing the mitogen-activated protein kinase pathway.
Low let-7d microRNA levels in chick embryos enhance innate immunity against Mycoplasma gallisepticum by suppressing the mitogen-activated protein kinase pathway.
复制标题
DOI：
10.1186/s13567-023-01178-6
复制
发表时间：
2023-06-19
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
鸡胚胎是研究免疫和疫苗的重要模型。因此，研究鸡毒支原体（MG）诱导鸡胚免疫反应的分子机制对于MG的预防和控制至关重要。在本研究中，我们在感染 MG 的鸡胚肺中筛选下调的 let-7d microRNA，以探讨其参与针对 MG 的先天免疫机制。在这里，我们证明低水平的let-7d是MG存在下鸡胚原代II型肺细胞（CP-II）的保护机制。具体来说，我们发现 CP-II 细胞中 let-7 水平降低会降低 MG 的粘附能力。这种抑制作用是通过激活丝裂原激活蛋白激酶磷酸酶 1 (MKP1) 靶基因和失活丝裂原激活蛋白激酶 (MAPK) 途径来实现的。此外，下调let-7d 可以减轻 MG 诱导的过度炎症和细胞死亡。总之，鸡胚通过先天免疫分子let-7d保护自身免受MG感染，这可能是由于其作为MAPK通路抑制剂的功能，有效减轻MG粘附、炎症反应和细胞凋亡。这项研究可能为重症肌无力疫苗的开发提供新的见解。
Chick embryos are a valuable model for studying immunity and vaccines. Therefore, it is crucial to investigate the molecular mechanism of the Mycoplasma gallisepticum (MG)-induced immune response in chick embryos for the prevention and control of MG. In this study, we screened for downregulated let-7d microRNA in MG-infected chicken embryonic lungs to explore its involvement in the innate immune mechanism against MG. Here, we demonstrated that low levels of let-7d are a protective mechanism for chicken embryo primary type II pneumocytes (CP-II) in the presence of MG. Specifically, we found that depressed levels of let-7 in CP-II cells reduced the adhesion capacity of MG. This suppressive effect was achieved through the activated mitogen-activated protein kinase phosphatase 1 (MKP1) target gene and the inactivated mitogen-activated protein kinase (MAPK) pathway. Furthermore, MG-induced hyperinflammation and cell death were both alleviated by downregulation of let-7d. In conclusion, chick embryos protect themselves against MG infection through the innate immune molecule let-7d, which may result from its function as an inhibitor of the MAPK pathway to effectively mitigate MG adhesion, the inflammatory response and cell apoptosis. This study may provide new insight into the development of vaccines against MG.
登录
查看更多内容
DOI：
10.4049/jimmunol.0804343
发表时间：
2009-12-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Frazier WJ;Wang X;Wancket LM;Li XA;Meng X;Nelin LD;Cato AC;Liu Y
通讯作者：
Liu Y
影响因子：
4
作者：
Awad, N. F. S.;Abd El-Hamid, M. I.;Mahmoud, H. I.
通讯作者：
Mahmoud, H. I.
鸡毒支原体感染对鸡肺能量代谢的影响：通过氧化应激和炎症
影响因子：
3.8
作者：
Ishfaq, Muhammad;Zhang, Wei;Li, Jichang
通讯作者：
Li, Jichang
影响因子：
2.1
作者：
Candas, Demet;Li, Jian Jian
通讯作者：
Li, Jian Jian
影响因子：
4.9
作者：
Liu D;Du J;Sun J;Li M
通讯作者：
Li M