Production of endothelin by canine prostatic epithelial cells and its stimulatory effects on their growth.
Production of endothelin by canine prostatic epithelial cells and its stimulatory effects on their growth.
复制标题
犬前列腺上皮细胞产生内皮素及其对其生长的刺激作用。
作者：
Kazuhiro Ishizaka;Hiroshi Azuma;Osamu Matsubara;Satoshi Kttahara;Hiroyuki Oshima
本研究旨在探讨犬前列腺中内皮素（ET）受体和ET的表达以及ET对犬前列腺上皮细胞的影响。 ET 受体的特征是前列腺冰冻切片中生物素化的 ET-1 结合。原代培养中的犬前列腺上皮细胞用于通过反相HPLC结合放射免疫测定和Northern印迹来证明ET-1表达，并进行生长测定。生物素化的 ET-1 结合位于上皮成分中，并且这种结合也被 ET(B) 亚型受体特异性拮抗剂阻断。 ET-1和ET-3在体外刺激犬前列腺上皮细胞生长。当存在 ET(B) 亚型受体特异性拮抗剂时，该效应也会逆转。上皮细胞培养基的洗脱曲线显示两个峰，对应于ET-1和大ET-1。培养物中的上皮细胞表达前-ET-1原mRNA。犬前列腺上皮细胞表达 ET(B) 受体和 ET-1。表达的 ET-1 很可能通过 ET(B) 受体在犬前列腺上皮细胞上充当自分泌/旁分泌增殖因子。
The current study has been designed to explore expressions of endothelin (ET) receptors and ETs in the canine prostate and the effect of ETs on canine prostatic epithelial cells. ET receptors were characterized by biotinylated ET-1 binding in frozen sections of the prostate. Canine prostatic epithelial cells in primary culture were used for demonstration of ET-1 expression by reverse-phase HPLC coupled with radioimmunoassay and Northern blotting and were subjected to growth assay. Biotinylated ET-1 binding was localized in the epithelial component, and the binding was also blocked with an antagonist specific for ET(B) subtype receptors. ET-1 and ET-3 stimulated canine prostatic epithelial cell growth in vitro. The effect was also reversed in the presence of an antagonist specific for ET(B) subtype receptors. Elution profile of epithelial cell culture medium revealed two peaks, corresponding to ET-1 and big ET-1. Epithelial cells in culture expressed pre-pro-ET-1 mRNA. Canine prostatic epithelial cells expressed ET(B) receptors and ET-1. It appears most likely that the expressed ET-1 acts as an autocrine/paracrine proliferative factor on canine prostatic epithelial cells via ET(B) receptors.
使用赖氨酸 9 侧链生物素化的内皮素 1 评估内皮素受体群体。
DOI：
10.1016/0006-291x(91)92072-r
发表时间：
1991
影响因子：
3.1
作者：
Magazine,HI;Andersen,TT;Goligorsky,MS;Malik,AB
通讯作者：
Malik,AB