Mixtures of supported and hybrid lipid membranes on heterogeneously modified silica nanoparticles.
Mixtures of supported and hybrid lipid membranes on heterogeneously modified silica nanoparticles.
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DOI：
10.1021/jp308305y
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发表时间：
2013-02-21
影响因子：
3.3
通讯作者：
Reed, Scott M.
中科院分区：
文献类型：
--
作者：
Piper-Feldkamp, Aundrea R.;Wegner, Maria;Brzezinski, Peter;Reed, Scott M.
简单支持的脂质双层不能准确反映细胞膜的复杂异质性；然而，表面修饰使得调整膜特性以更好地模拟生物系统成为可能。在此，二氧化硅改性剂 3-[2-(2-氨基乙基氨基)乙基氨基]丙基三甲氧基硅烷 (DETAS) 促进了二氧化硅纳米颗粒上支撑脂质双层的形成。稳定支撑双层的证据来自于间隙水层内可溶性荧光团的成功捕获。利用成孔肽的荧光猝灭测定被用来证明两个独立的脂质小叶的存在。在此测定中，在添加蜂毒肽之前和之后，荧光被大致相等比例的连二亚硫酸盐猝灭。当疏水改性剂十八烷基三乙氧基硅烷与DETAS共沉积在纳米粒子上时，纳米粒子上负载的双层的量减少，杂化膜的量增加。这允许在单个基底上控制两种不同类型的膜的混合，一种由水垫隔开，另一种直接固定在表面上，从而提供了一种新的细胞膜模拟物。
Simple supported lipid bilayers do not accurately reflect the complex heterogeneity of cellular membranes; however, surface modification makes it possible to tune membrane properties to better mimic biological systems. Here, 3-[2-(2-aminoethylamino)ethylamino]propyl-trimethoxysilane (DETAS), a silica modifier, facilitated formation of supported lipid bilayers on silica nanoparticles. Evidence for a stable supported bilayer came from the successful entrapment of a soluble fluorophore within an interstitial water layer. A fluorescence-quenching assay that utilized a pore-forming peptide was used to demonstrate the existence of two separate lipid leaflets. In this assay, fluorescence was quenched by dithionite in roughly equal proportions prior to and after addition of melittin. When a hydrophobic modifier, octadecyltriethoxysilane, was co-deposited on the nanoparticles with DETAS, there was a decrease in the amount of supported bilayer on the nanoparticles and an increase in the quantity of hybrid membrane. This allowed for a controlled mixture of two distinct types of membranes on a single substrate, one separated by a water cushion and the other anchored directly on the surface, thereby providing a new mimic of cellular membranes.
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