A new method for continual quantitation of viable cells on endothelialized polyurethanes.
A new method for continual quantitation of viable cells on endothelialized polyurethanes.
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一种连续定量内皮化聚氨酯上活细胞的新方法。
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
P. Lelkes
中科院分区：
文献类型：
--
作者：
V. Nikolaychik;M. M. Samet;P. Lelkes
目前用于心血管假体的许多分段聚氨酯要么对其表面结构进行改性，要么内衬融合单层内皮细胞，以改善其血液相容性。在这些生物聚合物上建立内皮细胞衬里的过程中，有必要连续监测覆盖基底的活细胞的数量。然而，并不是所有的常规细胞计数技术都适用于评估聚氨酯上内皮细胞的生长。诸如直接细胞计数、染料摄取或DNA或蛋白质染色的方法需要透明的支架或导致在测量之前终止培养过程。此外，一些光谱测定常常受到染料和/或增溶剂与各种成分（例如，催化剂、抗氧化剂）和/或聚氨酯制剂中的官能团。在解决这些问题，我们采用了一种新的，高度可重复的荧光检测，这是基于减少由活细胞的电化学敏感的化合物，Alcohol蓝。生物还原产物在培养基中可溶且稳定，无细胞毒性。此外，该测定与聚合物表面的几何形状或物理化学性质无关。在本研究中，我们专注于实施本试验，以监测不同类型的内皮细胞在分段聚氨酯上的附着和生长。
Many of the segmented polyurethanes currently used in cardiovascular prostheses undergo either modification of their surface structure or are lined with a confluent monolayer of endothelial cells to improve their hemocompatibility. During the establishment of an endothelial cell lining on these biopolymers it is necessary to continually monitor the number of viable cells that are covering the substrate. Yet, not all of the conventional cell enumeration techniques are suitable for assessing the growth of endothelial cells on polyurethanes. Methods, such as direct cell counting, dye uptake, or DNA or protein staining require either a transparent scaffold or lead to termination of the culturing process prior to measurement. In addition, some of the spectroscopic assays are often hampered by interaction of the dyes and/or solubilizers with the various constituents (e.g., catalyzers, antioxidants) and/or functional groups in the polyurethane formulations. In addressing these problems, we adapted a novel, highly reproducible fluorescent assay which is based on reduction by viable cells of an electrochemically sensitive compound, Alamar Blue. The bioreduced product is soluble and stable in culture media and noncytotoxic. In addition, the assay is independent of the geometry or physicochemical properties of the polymeric surfaces. In the present study we focus on the implementation of this assay to monitoring attachment and growth of various endothelial cell types on segmented polyurethanes.
牛主动脉内皮细胞在添加剂改性的聚（醚型聚氨酯脲）上的附着和增殖。
DOI：
10.1002/jbm.820270409
发表时间：
1993
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Brunstedt,MR;Ziats,NP;Rose-Caprara,V;Hiltner,PA;Anderson,JM;Lodoen,GA;Payet,CR
通讯作者：
Payet,CR
小口径血管移植物的体外内皮化。
影响因子：
3.8
作者：
Anderson,JS;Price,TM;Hanson,SR;Harker,LA
通讯作者：
Harker,LA
DOI：
10.1002/jbm.820280307
发表时间：
1994-03-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
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作者：
LIN, HB;SUN, W;COOPER, SL
通讯作者：
COOPER, SL