Attachment and proliferation of bovine aortic endothelial cells onto additive modified poly(ether urethane ureas).
Attachment and proliferation of bovine aortic endothelial cells onto additive modified poly(ether urethane ureas).
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牛主动脉内皮细胞在添加剂改性的聚（醚型聚氨酯脲）上的附着和增殖。
DOI：
10.1002/jbm.820270409
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Payet,CR
中科院分区：
文献类型：
--
作者：
Brunstedt,MR;Ziats,NP;Rose-Caprara,V;Hiltner,PA;Anderson,JM;Lodoen,GA;Payet,CR
为了更好地了解内皮细胞与聚醚脲（PEUU）材料的相互作用，并评估牛主动脉内皮细胞的附着，将膜与BAEC在含有5%胎牛血清的培养基中孵育24小时。其余膜在不含细胞的5%胎牛血清培养基中再孵育4天，以评估BAEC的增殖情况。该实验是在丙烯酸酯和甲基丙烯酸酯聚合物和共聚物添加剂改性的PEUU薄膜上进行的，这些添加剂在疏水性/亲水性尺度上跨越了很宽的范围。以组织培养聚苯乙烯（TCPS）为对照。实验表明，与未加载的PEUU膜（附着73%，增殖47%）或加载疏水丙烯酸酯或甲基丙烯酸酯聚合物添加剂的PEUU膜(附着32-69%；增殖,18 - 57%)。实验还表明，涂有均聚（二异丙基丙烯酸酯）（h‐DIPAA）的PEUU膜与涂有h‐癸基丙烯酸酯（h‐DA）的PEUU膜相比，能显著增强BAEC的附着和增殖；涂覆这两种丙烯酸酯共聚物（即co‐[DIPAA/DA][3/1]）的薄膜表现出中间行为。为了解释与未改性的PEUU膜或含有疏水性丙烯酸酯和甲基丙烯酸酯聚合物添加剂的PEUU膜相比，含有甲基丙烯酸酯2138F或h - DIPAM的膜增强了BAEC的相互作用，我们假设在膜形成过程中，靠近溶剂膨胀PEUU基质表面区域的添加剂可能迁移到或靠近PEUU -空气界面，从而形成了一个添加剂富集的PEUU表面区域。这表明，一旦在这个表面区域，响应于水介质的动态重定向确保了添加剂能够显著影响蛋白质吸附和伴随的内皮细胞行为，但前提是它们与水介质的相互作用比PEUU更有利。甲基丙醇和h - DIPAM添加剂增强内皮细胞行为的能力被认为是亲水性增强的结果。这是由于暴露的氢键DIPAM部分和增加的表面柔韧性的结果，这本身是由于无阻碍的甲基丙醇链的水化，这可能会产生一个添加剂富集的PEUU -水界面区。©1993 John Wiley & Sons, Inc
To better understand endothelial cell interactions with poly(ether urethane urea) (PEUU) materials, and to assess bovine aortic endothelial cell attachment, films were incubated for 24 h with BAEC in media containing 5% fetal bovine serum. Other films were allowed to incubate for 4 more days in media containing 5% fetal bovine serum without cells to assess BAEC proliferation. The assay was performed on PEUU films modified with acrylate and methacrylate polymer and copolymer additives that spanned a wide range on the hydrophobicity/hydrophilicity scale. Tissue culture polystyrene (TCPS) was used as a control. The assay showed that PEUU films loaded with Methacrol 2138F [copoly(diisopropylaminoethyl methacrylate][DIPAM]/decyl methacrylate [DM]) (3/1) or with its hydrophilic component, DIPAM, in homopolymer form (i. e., h‐DIPAM), significantly enhanced BAEC attachment (∼80% of TCPS values) and proliferation (∼80%) when compared to unloaded PEUU films (attachment, 73%; proliferation, 47%) or to PEUU films loaded with the more hydrophobic acrylate or methacrylate polymer additives (attachment, 32–69%; proliferation, 18–57%). The assay also showed that PEUU films coated with homopoly(diisopropylaminoethyl acrylate) (h‐DIPAA) significantly enhanced BAEC attachment and proliferation when compared to PEUU films coated with h‐decyl acrylate (h‐DA); films coated with the copolymer of these two acrylates (i.e., co‐[DIPAA/DA] [3/1]) showed intermediate behavior. To explain the enhancement of BAEC interaction with films loaded with Methacrol 2138F or h‐DIPAM, when compared to unmodified PEUU films or to PEUU films loaded with more hydrophobic acrylate and methacrylate polymer additives, it was assumed that the additives near the surface region of the solvent swollen PEUU matrix may have migrated to, or near to, the PEUU‐air interface during film formation, creating an additive enriched PEUU surface region. It is suggested that, once at this surface region, dynamic reorientation in response to an aqueous medium ensured the additives were able significantly to influence protein adsorption, and concomitant endothelial cell behavior, but only if they interacted with aqueous media more favorably than the PEUU. The ability of Methacrol and h‐DIPAM additives to enhance endothelial cell behavior is argued to be the result of increased hydrophilicity. This is the result of exposed, hydrogen‐bonding DIPAM moieties and increased surface flexibility, which is itself due to the hydration of unhindered Methacrol chains, which may create an additive enriched PEUU‐water interfacial zone. © 1993 John Wiley & Sons, Inc.
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DOI：
10.1001/archsurg.1987.01400160054007
发表时间：
1987
期刊：
Archives of surgery (Chicago, Ill. : 1960)
影响因子：
--
作者：
Hasson,JE;Wiebe,DH;Abbott,WM
通讯作者：
Abbott,WM
DOI：
10.1002/jbm.820220403
发表时间：
1988-04-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
ABSOLOM, DR;HAWTHORN, LA;CHANG, G
通讯作者：
CHANG, G
影响因子：
14
作者：
LYDON, MJ;MINETT, TW;TIGHE, BJ
通讯作者：
TIGHE, BJ
影响因子：
14
作者：
KLEINSOYER, C;HEMMENDINGER, S;CAZENAVE, JP
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影响因子：
4.3
作者：
P. Zilla;R. Fasol;M. Deutsch;T. Fischlein;E. Minar;A. Hammerle;Olga Krapicka;M. Kadletz
通讯作者：
M. Kadletz